Roos R W, Lau-Cam C A
J Pharm Sci. 1985 Feb;74(2):201-4. doi: 10.1002/jps.2600740220.
A high-performance liquid chromatographic (HPLC) method was developed for the analysis of conjugated and esterified estrogens in tablets. The 3-sulfate estrogen derivatives were hydrolyzed with acid, mixed with ethinyl estradiol (the internal standard), and extracted into benzene. The liberated 3-phenolic estrogens were converted to dansyl derivatives, separated on an adsorption column, and detected fluorometrically. Estrogens with a 17-keto functional group required a prior reduction to the corresponding 17 beta-hydroxyl derivatives with sodium borohydride in order to be chromatographically resolved. The validity of the proposed method was demonstrated by analyzing samples of commercial tablets of conjugated and esterified estrogens of various strengths.
建立了一种高效液相色谱（HPLC）法，用于分析片剂中的结合型和酯化型雌激素。将3-硫酸酯雌激素衍生物用酸水解，与炔雌醇（内标）混合，并用苯萃取。释放出的3-酚羟基雌激素转化为丹磺酰衍生物，在吸附柱上分离，并用荧光法检测。具有17-酮官能团的雌激素需要先用硼氢化钠预先还原为相应的17β-羟基衍生物，以便进行色谱分离。通过分析不同强度的结合型和酯化型雌激素商业片剂样品，证明了该方法的有效性。
