Roos R W, Medwick T
J Chromatogr Sci. 1979 Nov;17(11):624-7. doi: 10.1093/chromsci/17.11.624.
The chromatographic behavior of the estrogen carbonyls, equilenin, equilin, and estrone, and their respective sodium borohydride reduction products has been studied. The separation of the 17 alpha-hydroxy reduced compounds is superior to the separations achieved for the parent carbonyls using both reversed-phase and normal-phase systems. The reductions appear quantitative by the chromatographic systems used but other work indicates that a small quantity of 17 alpha-hydroxy isomer is produced. The separations developed here have been used in the identification of 9-dehydroestrone, an impurity in estrone, and in the identification of the estrogens in a commercial aqueous suspension of estrogenic substances.
已对雌激素羰基化合物、马萘雌酮、马烯雌酮和雌酮及其各自的硼氢化钠还原产物的色谱行为进行了研究。使用反相和正相系统时，17α-羟基还原化合物的分离效果优于母体羰基化合物的分离效果。在所使用的色谱系统中，还原反应似乎是定量的，但其他研究表明会生成少量的17α-羟基异构体。此处开发的分离方法已用于鉴定雌酮中的杂质9-脱氢雌酮，以及鉴定商业雌激素物质水悬浮液中的雌激素。
