Ojima Yoshihiro, Akiyoshi Riho, Tokiwa Itto, Nakazono Takashi, Yamada Yusuke, Azuma Masayuki
Department of Chemistry and Bioengineering, Osaka Metropolitan University, 3-3-138, Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
Research Center for Artificial Photosynthesis, Osaka Metropolitan University, 3-3-138, Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
Biotechnol Rep (Amst). 2025 Jan 9;45:e00874. doi: 10.1016/j.btre.2025.e00874. eCollection 2025 Mar.
A phosphorus (P)-accumulating mutant strain of is used as a precursor for transition metal phosphides (TMPs) biochar. Tetrahydrofuran treatment of the P-accumulating yeast coupled with pyrolysis resulted in the formation of CoP-loaded biochar (CoP@P-yeast) unlike previously reported CoP-loaded biochar using dry baker's yeast. The CoP@P-yeast exhibited the electrocatalytic activity for the hydrogen evolution with an overpotential of -192 mV at 10 mA cm. Furthermore, the CoP@P-yeast showed the highest ammonia production rate of 33 mg-NH h mg-catalyst in nitrate reduction reaction, as well as much higher than that with platinum on graphitized carbon. Scanning electron microscopy and transmission electron microscopy observations revealed that relatively large TMP crystals mainly located at the biochar surface, which may be beneficial to avoid catalytic deterioration during the nitrate reduction reaction. This study demonstrates that P-accumulating mutant strain of yeast is a suitable precursor to improve the activity of the resulting TMP biochar.
一种磷（P）积累突变酵母菌株被用作过渡金属磷化物（TMPs）生物炭的前驱体。与使用干面包酵母制备负载CoP的生物炭不同，对积累磷的酵母进行四氢呋喃处理并结合热解，得到了负载CoP的生物炭（CoP@P-酵母）。CoP@P-酵母在10 mA cm时对析氢具有电催化活性，过电位为-192 mV。此外，CoP@P-酵母在硝酸盐还原反应中显示出最高的氨生成速率，为33 mg-NH h mg-催化剂，也远高于石墨化碳上负载铂的情况。扫描电子显微镜和透射电子显微镜观察表明，相对较大的TMP晶体主要位于生物炭表面，这可能有利于避免硝酸盐还原反应过程中的催化性能恶化。这项研究表明，酵母的磷积累突变菌株是提高所得TMP生物炭活性的合适前驱体。
