Boualam M A, Bouri A, Signoli M, Drancourt M, Caputo A, Terrer E, Aboudharam G
Aix-Marseille University, MEPHI, AP-HM, IHU Méditerranée Infection, Marseille, France.
IHU Méditerranée Infection, Marseille, France.
Curr Res Microb Sci. 2025 Jan 9;8:100339. doi: 10.1016/j.crmicr.2025.100339. eCollection 2025.
() bacteraemia is a rare situation, with only four case reports in the literature. Fourteen teeth from the 1590 plague site in Fédons, France, were surface decontaminated before the pulp was cultured under strict anaerobiosis with negative controls. Colonies were identified by mass spectrometry and whole genome sequencing, and specific PCR was performed using DNA extracted from dental pulps, calculus and sediments. cultured in two dental pulp specimens from two individuals was firmly identified by MALDI-TOF mass spectrometry, and whole genome sequencing confirmed toxigenic . In the remaining twelve individuals, no such was recovered and further detection by PCR and palaeoculturomics of dental calculus and sediments surrounding the teeth in these two individuals remained negative. Toxigenic which did not result from mere environmental contamination, caused bacteraemia in two individuals from a modern time plague site in France.
（）菌血症是一种罕见情况，文献中仅有4例病例报告。法国费东1590年鼠疫遗址的14颗牙齿在牙髓培养前进行了表面去污处理，牙髓在严格厌氧条件下培养，并设置了阴性对照。通过质谱和全基因组测序鉴定菌落，并使用从牙髓、牙石和沉积物中提取的DNA进行特异性PCR。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）在两名个体的两个牙髓标本中培养出的菌落得到了明确鉴定，全基因组测序证实了其产毒性。在其余12名个体中，未检测到此类菌，对这两名个体牙齿周围的牙石和沉积物进行PCR和古培养组学进一步检测结果仍为阴性。并非单纯由环境污染导致的产毒性菌在法国一个现代鼠疫遗址的两名个体中引起了菌血症。
