Spinelli Veronica, Ceci Andrea, Giovannini Roberto, Sciubba Fabio, Persiani Anna Maria
Department of Environmental Biology, Sapienza University of Rome, Rome, Italy.
NMR-Based Metabolomics Laboratory (NMLab), Sapienza University of Rome, Rome, Italy.
Mycologia. 2025 Mar-Apr;117(2):331-345. doi: 10.1080/00275514.2024.2445759. Epub 2025 Feb 3.
In this study, and and their metabolites were assessed in vitro for their ability to inhibit growth of , and , gaining insights into their biocontrol mechanisms. A dual culture, an assay for volatile antimicrobial compounds effectiveness (performed in two different conditions), and a culture filtrate antifungal assay were designed to discriminate the involved mechanisms. Moreover, the culture filtrates of these strains were assessed for fungistatic and fungicidal activities (determining also the minimum inhibitory concentration and the minimum fungicidal concentration) and for the occurrence of siderophores. The results show that both and inhibited, to different extents, growth of all the pathogens in the plate assays. Both culture filtrates showed fungistatic and fungicidal activities, pointing to the release of diffusible compounds as an involved biocontrol mechanism. Based on the results of this study, and are promising bioprotection agents of these phytopathogens and species of interest for further studies aimed at validating their potential in in vivo conditions.
在本研究中，对[具体物质1]、[具体物质2]及其代谢产物进行了体外评估，以确定它们抑制[病原菌1]、[病原菌2]和[病原菌3]生长的能力，从而深入了解其生物防治机制。设计了双培养、挥发性抗菌化合物有效性测定（在两种不同条件下进行）以及培养滤液抗真菌测定，以区分其中涉及的机制。此外，还评估了这些菌株的培养滤液的抑菌和杀菌活性（同时确定最低抑菌浓度和最低杀菌浓度）以及铁载体的产生情况。结果表明，在平板试验中，[具体物质1]和[具体物质2]均不同程度地抑制了所有病原菌的生长。两种培养滤液均表现出抑菌和杀菌活性，表明可扩散化合物的释放是一种涉及的生物防治机制。基于本研究结果，[具体物质1]和[具体物质2]是这些植物病原菌有前景的生物保护剂，也是进一步研究以验证其在体内条件下潜力的目标物种。
