Barnes Denicia, Hoke Allison, Hammamieh Rasha, Gautam Aarti
Medical Readiness Systems Biology, CMPN, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Oak Ridge Institute of Science and Education (ORISE), Oak Ridge, TN, USA.
Methods Mol Biol. 2025;2880:1-16. doi: 10.1007/978-1-0716-4276-4_1.
Saliva, a readily available and noninvasive biofluid, has emerged as a promising source for gene expression studies, offering a window into both local and systemic gene expression patterns. The salivary transcriptome and miRNome hold valuable information about the physiological and pathological processes occurring in the oral cavity and throughout the body.This chapter delves into the potential of saliva as a noninvasive sampling method, exploring its utility in gene expression profiling for various applications. It provides an overview of the components contributing to the salivary transcriptome and discusses the challenges associated with salivary RNA analysis. We highlight the applications of salivary gene expression studies in biomarker discovery for oral and systemic diseases.While discussing various saliva collection techniques, here we focus on the procedure for RNA extraction, including microRNA (miRNA) from the OMNIgene™ SALIVA DNA and RNA device, OMR-610 (DNA Genotek Inc., Ottawa, Ontario, Canada). Herein, we provide the detailed methodologies for RNA extraction for salivary transcriptomics and the miRNome, thus providing a resource for researchers interested in leveraging the diagnostic and prognostic potential of saliva for personalized medicine and precision health initiatives.
唾液是一种易于获取的非侵入性生物流体，已成为基因表达研究的一个有前景的来源，为了解局部和全身基因表达模式提供了一个窗口。唾液转录组和微小RNA组包含有关口腔和全身发生的生理和病理过程的宝贵信息。本章深入探讨唾液作为一种非侵入性采样方法的潜力，探索其在各种应用的基因表达谱分析中的效用。它概述了构成唾液转录组的成分，并讨论了与唾液RNA分析相关的挑战。我们强调唾液基因表达研究在口腔和全身疾病生物标志物发现中的应用。在讨论各种唾液收集技术时，我们在此重点关注RNA提取过程，包括从OMNIgene™ SALIVA DNA和RNA装置OMR-610（加拿大安大略省渥太华的DNA Genotek公司）中提取微小RNA（miRNA）。在此，我们提供唾液转录组学和微小RNA组RNA提取的详细方法，从而为有兴趣利用唾液的诊断和预后潜力开展个性化医疗和精准健康计划的研究人员提供资源。
