Cheng Xiaoqing, Yun Xing, Wei Yu, Shi Pengtao, He Ruichao, Yang Chen, Liao Lujian, Wei Min, Quan Qi
Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai 200241, China.
Department of Orthopedic Surgery, the Fourth Medical Center of Chinese PLA General Hospital, Beijing 100142, China.
ACS Appl Mater Interfaces. 2025 Feb 12;17(6):8937-8948. doi: 10.1021/acsami.4c18082. Epub 2025 Feb 3.
Tendon, a connective tissue structure, serves the crucial role of transmitting force between muscles and bones. However, tendon injury repair continues to pose a significant challenge in clinical settings. In this study, we utilized single-cell RNA sequencing to delve into the cell populations and signaling pathways that are integral to tendon healing. Our findings suggest that hypoxia plays a pivotal role in activating macrophages, stimulating endothelial cell migration, and fostering fibroblast proliferation. Based on these insights, we have developed a PCL scaffold coated with DFOA, which effectively mimics a hypoxic environment to enhance tendon tissue regeneration. Furthermore, the PCL-DFOA scaffolds exhibit exceptional ability in promoting macrophage polarization and angiogenesis. This research offers a therapeutic strategy that harnesses the regenerative power of hypoxia to accelerate and optimize tendon healing processes.
肌腱是一种结缔组织结构，在肌肉和骨骼之间传递力量方面发挥着关键作用。然而，肌腱损伤修复在临床环境中仍然是一个重大挑战。在本研究中，我们利用单细胞RNA测序深入探究肌腱愈合所必需的细胞群体和信号通路。我们的研究结果表明，缺氧在激活巨噬细胞、刺激内皮细胞迁移和促进成纤维细胞增殖方面起着关键作用。基于这些见解，我们开发了一种涂有DFOA的聚己内酯（PCL）支架，该支架能有效模拟缺氧环境以促进肌腱组织再生。此外，PCL-DFOA支架在促进巨噬细胞极化和血管生成方面表现出卓越能力。这项研究提供了一种治疗策略，利用缺氧的再生能力来加速和优化肌腱愈合过程。
