Dong Wenyang, Shang Jiao, Guo Xinyu, Wang Haishan, Zhu Jiahao, Liang Pei, Shi Xueyan
Department of Entomology, China Agricultural University, Beijing 100193, China.
Department of Entomology, China Agricultural University, Beijing 100193, China.
Int J Biol Macromol. 2025 Apr;303:140634. doi: 10.1016/j.ijbiomac.2025.140634. Epub 2025 Feb 2.
Aphis gossypii Glover as a destructive agricultural pest has evolved resistance to various insecticides. Cycloxaprid is a novel structure neonicotinoid insecticide with excellent toxicity against A. gossypii. However, the resistance mechanism of A. gossypii to cycloxaprid was unclear. In the present study, a cycloxaprid-resistant (Cpd-R) strain (80.1-fold) of A. gossypii was obtained by continuous selection. Bioassay results showed that piperonyl butoxide significantly increased the toxicity of cycloxaprid by 10.5-fold to the Cpd-R strain. The activity of P450s was significantly higher in Cpd-R strain than in susceptible (Cpd-S) strain. The transcriptomic and qRT-PCR results showed that CYP6CY14, CYP380C44 and CYP303A1 were significantly upregulated in Cpd-R strain compared with Cpd-S strain. Furthermore, knockdown of CYP6CY14, CYP380C44 and CYP303A1 via RNA interference (RNAi) significantly increased the sensitivity of Cpd-R strain to cycloxaprid. Based on the higher expression of CYP6CY14 and RNAi results, transgenic Drosophila assay was conducted to further clarify the role of CYP6CY14 in cycloxaprid resistance, and results showed a significant increase in resistance to cycloxaprid in D. melanogaster. Additionally, the results of RNAi, dual-luciferase reporter and yeast one-hybrid (Y1H) indicated that CREB/ATF directly regulates CYP6CY14 expression. These findings provide necessary basis for clarifying the resistance mechanism of cycloxaprid in A. gossypii.
棉蚜作为一种具有破坏性的农业害虫，已对多种杀虫剂产生了抗性。环氧虫啶是一种新型结构的新烟碱类杀虫剂，对棉蚜具有优异的毒性。然而，棉蚜对环氧虫啶的抗性机制尚不清楚。在本研究中，通过连续筛选获得了棉蚜的环氧虫啶抗性（Cpd-R）品系（80.1倍）。生物测定结果表明，增效醚使环氧虫啶对Cpd-R品系的毒性显著提高了10.5倍。Cpd-R品系中P450s的活性显著高于敏感（Cpd-S）品系。转录组学和qRT-PCR结果表明，与Cpd-S品系相比，Cpd-R品系中CYP6CY14、CYP380C44和CYP303A1显著上调。此外，通过RNA干扰（RNAi）敲低CYP6CY14、CYP380C44和CYP303A1显著提高了Cpd-R品系对环氧虫啶的敏感性。基于CYP6CY14的高表达和RNAi结果，进行了转基因果蝇试验以进一步阐明CYP6CY14在环氧虫啶抗性中的作用，结果表明黑腹果蝇对环氧虫啶的抗性显著增加。此外，RNAi、双荧光素酶报告基因和酵母单杂交（Y1H）的结果表明，CREB/ATF直接调节CYP6CY14的表达。这些发现为阐明棉蚜对环氧虫啶的抗性机制提供了必要的依据。
