Han Seungwan, Yang Eun Mo, Hur Eun-Mi
Laboratory of Neuroscience, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea; BK21 FOUR Future Veterinary Medicine Leading Education & Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
Laboratory of Neuroscience, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea; BK21 FOUR Future Veterinary Medicine Leading Education & Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea; Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea; Comparative Medicine Disease Research Center, Science Research Center, Seoul National University, Seoul 08826, South Korea; Interdisciplinary Program in Neuroscience, College of Natural Sciences, Seoul National University, Seoul 08826, South Korea.
Mol Cells. 2025 Mar;48(3):100189. doi: 10.1016/j.mocell.2025.100189. Epub 2025 Feb 2.
The advent of recombinant adeno-associated viral (rAAV) vector-mediated gene delivery has accelerated the comprehensive analysis and manipulation of the nervous system owing to its ability to regulate gene expression in a spatiotemporal manner, thereby facilitating the study of brain physiology and the investigation of the pathophysiology of neurological disorders. Here, we provide a concise guide to stereotaxic gene delivery into the mouse brain using rAAV vectors. Key considerations for designing a customized rAAV vector are discussed, along with an overview of the surgical procedures of intracranial stereotaxic injection. This article aims to assist neuroscientists in establishing experimental setups for genetic manipulation in the mouse brain.
重组腺相关病毒（rAAV）载体介导的基因递送技术的出现，加速了对神经系统的全面分析和操纵，因为它能够以时空方式调节基因表达，从而促进脑生理学研究和神经疾病病理生理学研究。在此，我们提供一份使用rAAV载体向小鼠脑内进行立体定位基因递送的简明指南。我们将讨论设计定制rAAV载体的关键注意事项，并概述颅内立体定位注射的手术过程。本文旨在帮助神经科学家建立小鼠脑内基因操纵的实验装置。
