巴西圣卡塔琳娜州卡诺伊尼亚斯白耳负鼠（Didelphis albiventris）中克氏锥虫的检测1。
Detection of Trypanosoma cruzi in white-eared opossums (Didelphis albiventris) from Canoinhas, Santa Catarina State, Brazil1.
作者信息
Pontarolo Giane Helenita, Pedrassani Daniela, Kühl Luís Felipe, Campos Monique Paiva, Tirado Thais Cristina, Figueiredo Fabiano Borges, Vieira Thállitha Samih Wischral Jayme, Calchi Ana Cláudia, André Marcos Rogério, Vieira Rafael Felipe da Costa, Barros Filho Ivan Roque de
机构信息
Departamento de Medicina Veterinária, Universidade Federal do Paraná - UFPR, Curitiba, PR, Brasil.
Departamento de Medicina Veterinária, Universidade do Contestado - UnC, Canoinhas, SC, Brasil.
出版信息
Rev Bras Parasitol Vet. 2025 Feb 3;34(1):e017024. doi: 10.1590/S1984-29612025003. eCollection 2025.
Opossums are synanthropic animals that participate in the zoonotic transmission cycles. Chagas disease, a neglected tropical disease caused by Trypanosoma cruzi, affects many domestic and wild animals and humans worldwide. This study aimed to determine the occurrence of T. cruzi in free-ranging opossums in Canoinhas, Santa Catarina, Brazil. Fifty opossums (Didelphis albiventris) (33 captured and 17 road-killed) were evaluated using Nested-PCR assay. All tissue samples were negative (0/17). Eight of the 33 (24.24%; 95% CI:11.94-40,89%) blood samples were positive for T. cruzi. No significant associations were found between the sex (male/ female, p = 0.423), the trap area (rural/urban, p = 0.163), and positivity for T. cruzi in opossum blood samples. All samples showed 100% identity with T. cruzi (KF788250) isolated from Panstrongylus megistus in São Paulo, Brazil. The phylogenetic analysis model allocated all sequences obtained from D. albiventris to the large TcI clade of T. cruzi. This study provides the first record of T. cruzi in white-eared opossums in Canoinhas, Santa Catarina, southern Brazil.
负鼠是参与人畜共患病传播循环的共生动物。恰加斯病是一种由克氏锥虫引起的被忽视的热带疾病，影响着全球许多家养动物、野生动物和人类。本研究旨在确定巴西圣卡塔琳娜州卡诺伊尼亚斯自由放养的负鼠中克氏锥虫的感染情况。使用巢式PCR检测法对50只负鼠（白腹袋鼬）（33只捕获的和17只路杀的）进行了评估。所有组织样本均为阴性（0/17）。33份血液样本中有8份（24.24%；95%可信区间：11.94 - 40.89%）克氏锥虫呈阳性。在负鼠血液样本中，性别（雄性/雌性，p = 0.423）、诱捕区域（农村/城市，p = 0.163）与克氏锥虫阳性之间未发现显著关联。所有样本与从巴西圣保罗的巨蝽分离出的克氏锥虫（KF788250）显示100%的同一性。系统发育分析模型将从白腹袋鼬获得的所有序列归入克氏锥虫的大型TcI分支。本研究首次记录了巴西南部圣卡塔琳娜州卡诺伊尼亚斯白耳负鼠中克氏锥虫的感染情况。
Zotero 插件