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近红外光谱法作为鲜食葡萄无核分选的一种替代方法。

NIRS as an alternative method for table grapes Seedlessness sorting.

作者信息

Kanchanomai Chaorai, Theanjumpol Parichat, Maniwara Phonkrit, Kittiwachana Sila, Funsueb Sujitra, Ohashi Shintaroh, Naphrom Daruni

机构信息

Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand.

Department of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.

出版信息

MethodsX. 2025 Jan 11;14:103089. doi: 10.1016/j.mex.2024.103089. eCollection 2025 Jun.

DOI:10.1016/j.mex.2024.103089
PMID:39911907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11794173/
Abstract

Seedlessness in table grapes is a desirable trait for consumers. Plant growth regulators (PGRs) have been extensively utilized to induce seedlessness. However, the efficacy of these PGRs is not uniformly successful. In addition, the seedlessness is difficult to detect by cutting and counting technique. The shortwave-near infrared spectroscopy (SW-NIRS), coupled with suitable chemometric analysis, is a non-destructive method for sorting and prediction of seedlessness grapes. The NIRS is higher efficiency than original technique in term of accuracy, measuring time and waste reduction.•The SW-NIR spectra of 240 grape berries were recorded. Each reflectance spectrum was acquired in the wavenumber of 3996-12,489 cm. After that all grape berries were cut and count for seedlessness sorting.All spectral together with seedlessness sorting were be analysis by chemometrics.•The NIR spectral data were analyzed using principal component analysis (PCA). In addition, supervised self-organizing map (SSOM) and quadratic discriminant analysis (QDA) were applied to classify the seedlessness.•The PCA results represented a negative tendency to classify the seedlessness. Clear classification tendency can be obtained from SOMs. Good predictive results from SSOM were obtained, as it gave a percentage correctly classified of 97.14 and 94.64% for training and test sample sets, respectively.

摘要

鲜食葡萄的无核特性是消费者所期望的。植物生长调节剂(PGRs)已被广泛用于诱导无核。然而,这些植物生长调节剂的效果并不总是成功的。此外,通过切割和计数技术很难检测到无核情况。短波近红外光谱(SW-NIRS)结合合适的化学计量分析,是一种用于无核葡萄分选和预测的无损方法。在准确性、测量时间和减少浪费方面,近红外光谱技术比原始技术效率更高。

•记录了240个葡萄浆果的短波近红外光谱。每个反射光谱在3996 - 12489 cm的波数下采集。之后,将所有葡萄浆果切开并进行无核分选计数。所有光谱以及无核分选结果都通过化学计量学进行分析。

•使用主成分分析(PCA)对近红外光谱数据进行分析。此外,应用监督自组织映射(SSOM)和二次判别分析(QDA)对无核情况进行分类。

•主成分分析结果在对无核情况进行分类时呈现出负面趋势。自组织映射(SOMs)可以得到清晰的分类趋势。监督自组织映射(SSOM)获得了良好的预测结果,其训练样本集和测试样本集的正确分类百分比分别为97.14%和94.64%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d78b/11794173/7f8a5a7e01e0/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d78b/11794173/99f2e1d5491e/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d78b/11794173/7167c5d1ecd4/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d78b/11794173/c0a1b856e0a6/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d78b/11794173/7f8a5a7e01e0/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d78b/11794173/99f2e1d5491e/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d78b/11794173/7167c5d1ecd4/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d78b/11794173/c0a1b856e0a6/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d78b/11794173/7f8a5a7e01e0/gr3.jpg

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本文引用的文献

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GC-MS and HPLC-DAD analysis of fatty acid profile and functional phytochemicals in fifty cold-pressed plant oils in Thailand.泰国五十种冷榨植物油中脂肪酸谱和功能性植物化学物质的气相色谱-质谱联用(GC-MS)和高效液相色谱-二极管阵列检测(HPLC-DAD)分析
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Effect of GA3 treatment on seed development and seed-related gene expression in grape.
GA3 处理对葡萄种子发育和种子相关基因表达的影响。
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