zP-1噬菌体与氨苄西林对从医院工作人员中分离出的耐甲氧西林金黄色葡萄球菌的协同作用。

Synergistic effects of zP-1 phage and ampicillin against methicillin-resistant Staphylococcus aureus isolated from hospital staff.

作者信息

Mehmood Zain, Kanwar Rabia, Ullah Kaleem, Ali Saqib, Aslam Muhammad Aamir, Qadeer Abdul, Gul Haiwad, Mahmoud Mohamed Hassanein

机构信息

Faculty of Veterinary Science, Institute of Microbiology, University of Agriculture, Faisalabad, 38000, Pakistan.

Directorate General (Research) Livestock and Dairy Development Department, Peshawar, 25000, Khyber Pakhtunkhwa, Pakistan.

出版信息

Ir J Med Sci. 2025 Apr;194(2):611-621. doi: 10.1007/s11845-025-03898-4. Epub 2025 Feb 6.

DOI:10.1007/s11845-025-03898-4

PMID:39912979

Abstract

BACKGROUND

Methicillin-resistant Staphylococcus aureus (MRSA) strains are a major cause of hospital-acquired infections, exacerbated by increasing antibiotic resistance.

AIM

This study aimed to isolate bacteriophages targeting MRSA and evaluate their antimicrobial activity in combination with antibiotics.

METHODS

Nasal samples from hospital staff (n = 50) were used to isolate MRSA strains, and sewage samples were processed for phage isolation using the double agar overlay technique. The microtitration plate method evaluated the synergistic effects of isolated phages and antibiotics.

RESULTS

Out of 50 samples, 11 MRSA strains were positive, showing high resistance to multiple antibiotics, including oxacillin (95%), and vancomycin (85%). Phage zP-1, belonging to the Myoviridae family, exhibited > 90% lytic activity, stable across temperatures (20-50 °C) and pH (6-8). A statistically significant synergistic effect was observed at a MIC of 0.137 μg/ml for gentamicin (p-value 0.02).

CONCLUSION

These findings suggest that phage-antibiotic synergy may offer a promising strategy to combat MRSA infections, warranting further in vivo studies to assess its clinical potential.

摘要

背景

耐甲氧西林金黄色葡萄球菌（MRSA）菌株是医院获得性感染的主要原因，抗生素耐药性增加使情况更加恶化。

目的

本研究旨在分离靶向MRSA的噬菌体，并评估其与抗生素联合使用时的抗菌活性。

方法

使用医院工作人员的鼻腔样本（n = 50）分离MRSA菌株，并采用双层琼脂覆盖技术处理污水样本以分离噬菌体。微量滴定板法评估分离出的噬菌体与抗生素的协同作用。

结果

在50个样本中，11个MRSA菌株呈阳性，对多种抗生素表现出高度耐药性，包括苯唑西林（95%）和万古霉素（85%）。属于肌尾噬菌体科的噬菌体zP-1表现出> 90%的裂解活性，在温度（20 - 50°C）和pH（6 - 8）范围内稳定。庆大霉素MIC为0.137μg/ml时观察到具有统计学意义的协同效应（p值0.02）。