Vaccaro Giorgia, Stair Jacqueline L, Kirton Stewart B, Baker Daniel, Guirguis Amira
Department of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, UK.
School of Applied and Health Science, London South Bank University, London, UK.
Drug Test Anal. 2025 Sep;17(9):1607-1613. doi: 10.1002/dta.3864. Epub 2025 Feb 7.
Infusing synthetic cannabinoid receptor agonists on paper quickly became a main route for illicit entry into prisons (i.e., mail correspondence) in the last decade. So far, there are limited data on validated detection methods and typical concentration profiles of these substances on paper to inform interventions. An approach to quantify and map the synthetic cannabinoid receptor agonist (SCRA) 5F-MDMB-PINACA on a seized paper sample from a UK Prison was determined using ultraperformance liquid chromatography-mass spectrometry. The seized paper sample was initially screened using ultraperformance liquid chromatography-quadrupole time-of-flight-mass spectrometry that confirmed the presence of 5F-MDMB-PINACA. A quantification method was then optimised and validated for the SCRA using a simple liquid chromatography-quadrupole Dalton-mass spectrometry system. Percentage recovery studies were carried out on paper spiked with 1, 5 and 20 mg/cm of 5F-MDMB-PINACA with five consecutive MeOH extractions. Results showed that one extraction recovered 83%-86%, while three extractions resulted in 98%-99% 5F-MDMB-PINACA recovery. Finally, the method was used to determine the concentration profile across the seized paper sample; 5F-MDMB-PINACA was in detectable amounts on all the paper subunits (n = 39) ranging between 0.26 and 55.13 μg/cm, displaying a wide distribution of concentrations. Concentration profiles of SCRAs on seized paper samples are informative for interventions for those who are abusing or handling these substances in custodial settings but also provide key concentration ranges for those developing advanced methods of analysis in this area.
在过去十年中，在纸上注入合成大麻素受体激动剂迅速成为非法进入监狱的主要途径（即通过邮件通信）。到目前为止，关于这些物质在纸上的有效检测方法和典型浓度分布的数据有限，无法为干预措施提供依据。采用超高效液相色谱 - 质谱法确定了一种对从英国一所监狱查获的纸张样本中的合成大麻素受体激动剂5F - MDMB - PINACA进行定量和绘图的方法。最初使用超高效液相色谱 - 四极杆飞行时间质谱法对查获的纸张样本进行筛查，确认了5F - MDMB - PINACA的存在。然后使用简单的液相色谱 - 四极杆道尔顿质谱系统对该合成大麻素受体激动剂的定量方法进行了优化和验证。对添加了1、5和20mg/cm的5F - MDMB - PINACA的纸张进行了连续五次甲醇萃取的回收率研究。结果表明，一次萃取的回收率为83% - 86%，而三次萃取的5F - MDMB - PINACA回收率为98% - 99%。最后，该方法用于确定整个查获纸张样本的浓度分布；在所有纸张亚单位（n = 39）上均检测到5F - MDMB - PINACA，浓度范围在0.26至55.13μg/cm之间，显示出浓度分布广泛。查获纸张样本上合成大麻素受体激动剂的浓度分布对于在羁押场所滥用或处理这些物质的人进行干预具有参考价值，同时也为该领域开发先进分析方法的人提供了关键的浓度范围。
