Localization of agropine-synthesizing functions in the TR region of the root-inducing plasmid of Agrobacterium rhizogenes 1855.

The region of the Ri plasmid pRi 1855 that encodes agropine synthesis has been identified through its sequence homology with the equivalent genes of the octopine Ti plasmid pTi ACH5. Interestingly the agropine genes lie outside the so-far identified T-DNA of pRi 1855, and are separated from this latter by a long sequence of non integrated plasmid DNA. The presence of this additional T-DNA (TRight DNA) in hairy roots was demonstrated by Southern blot analysis and by the presence of specific transcripts. The genes for agropine synthesis are arranged in the Ri plasmid in a reversed order as compared to their orientation in the Ti plasmid pTi ACH5.