Lahners K, Byrne M C, Chilton M D
Plasmid. 1984 Mar;11(2):130-40. doi: 10.1016/0147-619x(84)90018-0.
Agrobacterium Ti (tumor-inducing) and Ri (root-inducing) plasmids transform dicot plant cells by insertion of a specific plasmid sector called T-DNA (transferred DNA) into host plant nuclear DNA. The mannopine -type Ri plasmid pRi8196 contains four BamHI fragments that encompass core T-DNA. We report Southern hybridization studies that show that these four fragments have no strong homology to octopine-, nopaline-, or agropine -type Ti plasmids. We detected and mapped very weak homology regions, most of which are assignable to opine synthase or opine catabolic functions on the Ti plasmid. We found no homology between Ri T-DNA and the region of Ti T-DNA that encodes tumor morphology functions.
根癌农杆菌Ti（致瘤）和Ri（发根）质粒通过将一个名为T-DNA（转移DNA）的特定质粒区段插入宿主植物核DNA中来转化双子叶植物细胞。甘露碱型Ri质粒pRi8196含有四个包含核心T-DNA的BamHI片段。我们报告了Southern杂交研究，结果表明这四个片段与章鱼碱型、胭脂碱型或农杆碱型Ti质粒没有强烈的同源性。我们检测并绘制了非常弱的同源区域，其中大部分可归因于Ti质粒上的章鱼碱合酶或章鱼碱分解代谢功能。我们发现Ri T-DNA与编码肿瘤形态功能的Ti T-DNA区域之间没有同源性。
