Vilaine F, Casse-Delbart F
Gene. 1987;55(1):105-14. doi: 10.1016/0378-1119(87)90253-8.
A new binary vector system has been constructed, based on agropine-type root-inducing plasmid (pRi) left transferred-region border sequences cloned in a plasmid containing the replication origin of another A. rhizogenes plasmid (pArA4a). This micro-pRi has been used to introduce a chimeric kanamycin resistance gene into tobacco plants, vir functions being provided by either octopine or nopaline tumor-inducing plasmids deleted of their own transferred regions. In addition, we show that cloning of pRi EcoRI fragment 15, which contains three open reading frames (which may correspond to loci rolA, B and C), in the micro-Ri vector generates a mini-pRi capable of inducing the proliferation of transformed roots.
构建了一种新的二元载体系统，该系统基于农杆碱型发根质粒（pRi）左转移区边界序列，这些序列克隆于一个含有另一种发根农杆菌质粒（pArA4a）复制起点的质粒中。这种微型pRi已被用于将嵌合卡那霉素抗性基因导入烟草植株，其vir功能由自身转移区缺失的章鱼碱或胭脂碱肿瘤诱导质粒提供。此外，我们还表明，将包含三个开放阅读框（可能对应于rolA、B和C位点）的pRi EcoRI片段15克隆到微型Ri载体中，可产生一种能够诱导转化根增殖的微型pRi。
