Birot A M, Casse-Delbart F
Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Versailles, France.
Plasmid. 1988 May;19(3):189-202. doi: 10.1016/0147-619x(88)90038-8.
Southern-type hybridizations were carried out in order to identify sequence homologies with the pTi vir loci, on an agropine-type plasmid (pRiHRI) and a mannopine-type plasmid (pRi8196) of Agrobacterium rhizogenes. The localization of the sequences hybridizing with subcloned fragments containing vir A, B, G, C, and D from pTiAch5 indicated a similar linear organization of the pTi vir loci and their homologies on pRiHRI and pRi8196, though no homology was detected on both pRi with a 1.1-kb internal fragment of virD. No homology was detected either with the vir E locus on pRiHRI vir region, nor with the virF locus on both pRi vir regions. As on nopaline pTiC58, fragments bearing the homologies with virC and virG are closer together on both pRi than on octopine pTiAch5. A preliminary functional map of the pRiHRI vir region is deduced from this study.
为了鉴定发根农杆菌的章鱼碱型质粒（pRiHRI）和甘露碱型质粒（pRi8196）上与pTi毒力基因座的序列同源性，进行了Southern杂交。与来自pTiAch5的包含vir A、B、G、C和D的亚克隆片段杂交的序列定位表明，pTi毒力基因座及其在pRiHRI和pRi8196上的同源性具有相似的线性组织，尽管在两个pRi上均未检测到与virD的1.1-kb内部片段的同源性。在pRiHRI毒力区域未检测到与vir E基因座的同源性，在两个pRi毒力区域也未检测到与virF基因座的同源性。与胭脂碱型pTiC58一样，在两个pRi上，与virC和virG具有同源性的片段比在章鱼碱型pTiAch5上更靠近。从这项研究中推导出了pRiHRI毒力区域的初步功能图谱。
