Yan Yajuan, Ding Lurong, Ding Jialian, Zhou Ping, Su Bin
Key Laboratory of Excited-State Materials of Zhejiang Province, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.
Chembiochem. 2024 Dec 2;25(23):e202400389. doi: 10.1002/cbic.202400389. Epub 2024 Aug 2.
Electrochemiluminescence (ECL) is one of the most powerful techniques that meet the needs of analysis and detection in a variety of scenarios, because of its highly analytical sensitivity and excellent spatiotemporal controllability. ECL combined with microscopy (ECLM) offers a promising approach for quantifying and mapping a wide range of analytes. To date, ECLM has been widely used to image biological entities and processes, such as cells, subcellular structures, proteins and membrane transport properties. In this review, we first introduced the mechanisms of several classic ECL systems, then highlighted the progress of visual biosensing and bioimaging by ECLM in the last decade. Finally, the characteristics of ECLM were summarized, as well as some of the current challenges. The future research interests and potential directions for the application of ECLM were also outlooked.
电化学发光（ECL）是满足各种场景下分析和检测需求的最强大技术之一，因为它具有高度的分析灵敏度和出色的时空可控性。电化学发光与显微镜技术相结合（ECLM）为定量和绘制各种分析物提供了一种很有前景的方法。迄今为止，ECLM已被广泛用于对生物实体和过程进行成像，如细胞、亚细胞结构、蛋白质和膜转运特性。在本综述中，我们首先介绍了几种经典ECL系统的机制，然后重点阐述了近十年来ECLM在可视化生物传感和生物成像方面的进展。最后，总结了ECLM的特点以及当前面临的一些挑战。还展望了ECLM未来的研究热点和潜在应用方向。
