T N Vipina Vinod, Mathew Amy S, Mathew Divya, Mathew Jyothis, E K Radhakrishnan
School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
Prep Biochem Biotechnol. 2025;55(7):855-868. doi: 10.1080/10826068.2025.2460498. Epub 2025 Feb 8.
Green synthesized nanomaterials play a vital role in nanotechnology was due to its diverse applications. In the current study, flower shaped nanoclusters of zinc oxide nanoparticles (ZnONPs) was fabricated using the leaf extract of (Linn.) by microwave-assisted method. The physico-chemical characterization of the green synthesized ZnONPs were further conducted by the UV-Vis spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and transmission electron microscopy analysis. The UV-Vis spectrum of the synthesized ZnONPs has showed characteristic absorption maximum at 363 nm. The XRD pattern of the same could confirm to have the crystalline nature of ZnONPs. Additionally, the FT-IR spectra have revealed the presence of characteristic stretching and bending vibrations of the Zn-O bond, along with those of phytochemicals that might have involved in ZnONPs stabilization. By the HR-TEM imaging, agglomeration of the nanoparticles and thereby the formation of flower-like clusters could be observed. Furthermore, the synthesized ZnONPs have remarkable antimicrobial activity against and with inhibition zones of 15 ± 0.4 and 16.5 ± 1.0 mm respectively. The green synthesized ZnONPs showed no significant toxicity toward . Hence, the results of the study indicate the promising potential of the synthesized ZnO nanoclusters.
绿色合成的纳米材料因其多样的应用在纳米技术中发挥着至关重要的作用。在当前研究中,采用微波辅助法,利用(林奈)的叶提取物制备了花状氧化锌纳米颗粒(ZnONPs)簇。通过紫外可见光谱、X射线衍射、傅里叶变换红外光谱、场发射扫描电子显微镜和透射电子显微镜分析对绿色合成的ZnONPs进行了进一步的物理化学表征。合成的ZnONPs的紫外可见光谱在363nm处显示出特征吸收最大值。其XRD图谱证实了ZnONPs的晶体性质。此外,FT-IR光谱揭示了Zn-O键的特征拉伸和弯曲振动的存在,以及可能参与ZnONPs稳定化的植物化学物质的振动。通过高分辨率透射电子显微镜成像,可以观察到纳米颗粒的团聚以及由此形成的花状簇。此外,合成的ZnONPs对和具有显著的抗菌活性,抑菌圈分别为15±0.4和16.5±1.0mm。绿色合成的ZnONPs对没有显示出明显的毒性。因此,该研究结果表明合成的ZnO纳米簇具有广阔的应用前景。