Xu Z, Tong Z, Zhang H, Deng X, Yang N, Wang Z, Chen C
Graduated from School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
These authors contributed equally to this work and were considered as the first authors.
Iran J Vet Res. 2024;25(3):224-232. doi: 10.22099/ijvr.2024.48969.7177.
outer membrane proteins (OMPs) are highly immunogenic, and lipopolysaccharides (LPS) are also considered significant antigens, making them potential candidates for subunit vaccines.
To investigate the effects of OMPs and LPSs on mouse bone marrow-derived dendritic cells (BMDC) activation and T-lymphocyte proliferation.
BMDC were isolated and cultured , and subsequently co-cultured with recombinant proteins (rOMP10, rOMP19, rBP26, rOMP25, and rOMP31), as well as smooth LPS (S-LPS) or rough LPS (R-LPS). The expression of maturation markers on the surface of BMDCs was determined using flow cytometry, while the expression of TLR receptors was determined using RT-PCR. The levels of inflammatory cytokines were measured using iELISA, and the impact on the proliferation of mouse T-lymphocytes was assessed using the MTT method.
The impact of LPS on BMDC maturation, TLRs-mediated cytokine secretion, and antigen presentation was found to be limited. In contrast, rOMP10, rOMP19, and rBP26 were observed to promote BMDC maturation, increase the expression of TLR-2 and TLR-4 mRNA, and activate T-lymphocyte proliferation by significantly increasing the expression of pro-inflammatory cytokines (TNF-α, IFN-γ, IL-6, IL-12) and antigen-presenting molecules. However, rOMP25 and rOMP31 did not promote BMDC maturation, inhibited the expression of MHCI and MHCII antigen-presenting molecules, and increased the expression of inflammation-suppressing cytokines (IL-10 and IL-4), resulting in the inhibition of T-lymphocyte proliferation.
OMP10, OMP19, and BP26 play an important role in activating the host immune response, while OMP25 together with OMP31 may play a role in immune escape.
外膜蛋白（OMPs）具有高度免疫原性，脂多糖（LPS）也被认为是重要抗原，使其成为亚单位疫苗的潜在候选物。
研究OMPs和LPSs对小鼠骨髓来源树突状细胞（BMDC）活化及T淋巴细胞增殖的影响。
分离培养BMDC，随后与重组蛋白（rOMP10、rOMP19、rBP26、rOMP25和rOMP31）以及光滑型LPS（S-LPS）或粗糙型LPS（R-LPS）共培养。采用流式细胞术检测BMDC表面成熟标志物的表达，采用RT-PCR检测TLR受体的表达。采用iELISA检测炎性细胞因子水平，采用MTT法评估对小鼠T淋巴细胞增殖的影响。
发现LPS对BMDC成熟、TLRs介导的细胞因子分泌及抗原呈递的影响有限。相比之下，观察到rOMP10、rOMP19和rBP26可促进BMDC成熟，增加TLR-2和TLR-4 mRNA的表达，并通过显著增加促炎细胞因子（TNF-α、IFN-γ、IL-6、IL-12）和抗原呈递分子的表达来激活T淋巴细胞增殖。然而，rOMP25和rOMP31未促进BMDC成熟，抑制MHC I和MHC II抗原呈递分子的表达，并增加抗炎细胞因子（IL-10和IL-4）的表达，从而导致T淋巴细胞增殖受到抑制。
OMP10、OMP19和BP26在激活宿主免疫反应中起重要作用，而OMP25与OMP一起可能在免疫逃逸中起作用。
