Mangal Prakhar, Sanikop Adarsh, Pandey Vikas, Satpathy Tanushree, Sharma Ripudaman, Sharma Shilpi, Patil Dipooja
Department of Oral Pathology and Microbiology, Maharana Pratap College of Dentistry and Research Centre, Gwalior, Madhya Pradesh, India.
Department of Pathology, Jawaharlal Nehru Medical College, Belagavi, Karnataka, India.
J Pharm Bioallied Sci. 2024 Dec;16(Suppl 4):S3467-S3469. doi: 10.4103/jpbs.jpbs_944_24. Epub 2024 Oct 4.
Oral squamous cell carcinoma (OSCC) is a prevalent malignancy characterized by varying histological grades. Basement membrane laminin expression plays a crucial role in tumor invasion and progression. This study aimed to assess the immunohistochemical expression of basement membrane laminin in different histological grades of OSCC.
Archival tissue samples of OSCC from 60 patients were categorized into well-differentiated, moderately differentiated, and poorly differentiated grades. Immunohistochemistry was performed using anti-laminin antibodies, and staining intensity was semiquantitatively analyzed using a scoring system.
The immunohistochemical analysis revealed varying levels of laminin expression across different histological grades of OSCC. Well-differentiated tumors showed an average staining intensity score of 7.2 (SD ± 1.5), moderately differentiated tumors had a score of 5.8 (SD ± 1.2), and poorly differentiated tumors exhibited a score of 4.3 (SD ± 0.9). Statistical analysis indicated a significant difference in laminin expression between histological grades ( < 0.001).
Our findings demonstrate a progressive decrease in basement membrane laminin expression with an increasing histological grade of OSCC. This suggests that laminin may be a potential biomarker for assessing tumor aggressiveness and prognosis in oral squamous cell carcinoma.
口腔鳞状细胞癌（OSCC）是一种常见的恶性肿瘤，具有不同的组织学分级。基底膜层粘连蛋白的表达在肿瘤侵袭和进展中起着关键作用。本研究旨在评估基底膜层粘连蛋白在不同组织学分级的OSCC中的免疫组化表达。
将60例OSCC患者的存档组织样本分为高分化、中分化和低分化等级。使用抗层粘连蛋白抗体进行免疫组化，并使用评分系统对染色强度进行半定量分析。
免疫组化分析显示，不同组织学分级的OSCC中层粘连蛋白表达水平不同。高分化肿瘤的平均染色强度评分为7.2（标准差±1.5），中分化肿瘤评分为5.8（标准差±1.2），低分化肿瘤评分为4.3（标准差±0.9）。统计分析表明，组织学分级之间层粘连蛋白表达存在显著差异（<0.001）。
我们的研究结果表明，随着OSCC组织学分级的增加，基底膜层粘连蛋白表达逐渐降低。这表明层粘连蛋白可能是评估口腔鳞状细胞癌肿瘤侵袭性和预后的潜在生物标志物。
