Al-Jumaa Zahraa Mustafa, Jaber Mohammed Tariq, Al-Doori Atheer Abdulrazzaq
Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Nineveh, Iraq.
Department of Forensic Biology, Higher Institute of Forensic Sciences, Al-Nahrain University, Baghdad, Iraq.
Open Vet J. 2024 Dec;14(12):3289-3295. doi: 10.5455/OVJ.2024.v14.i12.13. Epub 2024 Dec 31.
are obligate generally Gram-negative intracellular parasites with bacterial characteristics, including a cell wall, DNA, and RNA, and the main characteristics of infections are ocular conjunctivitis and upper respiratory disease.
The aim of this study was to investigate the prevalence of infection in a population of shelter cats in Baghdad.
Molecular detection was done using a polymerase chain reaction (PCR) approach. Fifty shelter cats of various ages, sexes, and breeds participated in the study from 1/11/2023 to 1/4/2024. DNA was extracted and amplified using PCR.
The study's findings revealed that the PCR technique showed that 22/44% of positive cats from a total of 50 cats were assured for the 23S rRNA gene and yielded a band at 400 bp, and for 41/27.3%, these findings are regarded as distinctive for the genus and positive sample 30/20% from a total of 150 samples was assured for , a band at 680 bp. The findings revealed that the prevalence of in upper respiratory tract infections in female cats older than 1 year was between 14/50 (28%) and 13/50 (26%) conversely, and the infections exhibited greater prevalence and a higher rate of detection in male under 1 year of age. The present investigation highlighted a significant prevalence of in respiratory swabs obtained from Persian and Himalayan cats, but Scottish and British cats exhibited a comparatively lower rate of positive .
In conclusion of this study, a significant number of cats are infected with , and PCR provided rapid and sensitive detection of in different samples and detected that did not grow in culture. It was considered the first study for the detection of from conjunctivitis in shelter cats in Baghdad.
是专性革兰氏阴性细胞内寄生虫，具有细菌特征，包括细胞壁、DNA和RNA，感染的主要特征是眼部结膜炎和上呼吸道疾病。
本研究旨在调查巴格达收容所猫群中感染的流行情况。
采用聚合酶链反应（PCR）方法进行分子检测。2023年11月1日至2024年4月1日，50只不同年龄、性别和品种的收容所猫参与了该研究。提取DNA并使用PCR进行扩增。
研究结果显示，PCR技术表明，在总共50只猫中，22只/44%的阳性猫的23S rRNA基因得到确认，在400 bp处产生条带；对于41只/27.3%的猫，这些结果被认为是该属的特征性结果，在总共150个样本中，30只/20%的阳性样本的条带在680 bp处得到确认。结果显示，1岁以上雌性猫上呼吸道感染中的感染率在14只/50只（28%）和13只/50只（26%）之间，相反，1岁以下雄性猫的感染率更高且检出率更高。本研究突出了从波斯猫和喜马拉雅猫的呼吸道拭子中检出的感染率较高，但苏格兰猫和英国猫的阳性率相对较低。
本研究的结论是，大量猫感染了，PCR能快速、灵敏地检测不同样本中的，还能检测出在培养中不生长的。这被认为是巴格达收容所猫结膜炎检测的第一项研究。
