Rout Sudeshna, Dash Rishikesh, Satish Varsha, Venugopal Giriprasad, Rao Bodepudi Narasimha, Bandhyopadhyay Debapriya, Bhoi Sanjeev Kumar, Ramadass Balamurugan
Department of Biochemistry, All India Institute of Medical Sciences Bhubaneswar, Odisha 751019, India.
Centre of Excellence for Clinical Microbiome and Research (CCMR), All India Institute of Medical Sciences Bhubaneswar, Odisha 751019, India.
Aging (Albany NY). 2025 Feb 7;17(2):393-407. doi: 10.18632/aging.206200.
With increased life expectancy, there is an increase in aging population and prevalence of dementia. Ghrelin is a key regulator of spatial memory and cognition. The gut microbiome may affect the circulating levels of unacylated ghrelin (UAG) and acylated ghrelin (AG). Thus, we explore the potential association of the gut microbiome, AG, and cognitive health in the aging dementia patient.
40 dementia patients and 40 controls were recruited. Fecal Microbiome analysis using 16S rRNA sequencing was performed on 18 samples. A mixed-method approach was employed for robust interpretation.
Dementia patients had an increased serum AG and AG/UAG ratio. With the increase in AG among dementia subjects, a significant decrease in species richness was observed. , and contributed to substantial differences in beta-diversity. was associated with Mini-Mental State Examination (MMSE), and was associated with Montreal Cognitive Assessment (MoCA) Scale.
This pilot study indicates a complex interaction between AG, gut microbiome, and cognitive scores. Increased AG corresponds to both dementia and gut dysbiosis, intricately interconnecting the gut-brain axis. The circulating AG and associated gut microbiome might be a putative biomarker for dementia.
随着预期寿命的增加，老年人口数量及痴呆症患病率也在上升。胃饥饿素是空间记忆和认知的关键调节因子。肠道微生物群可能会影响未酰化胃饥饿素（UAG）和酰化胃饥饿素（AG）的循环水平。因此，我们探讨了老年痴呆症患者肠道微生物群、AG与认知健康之间的潜在关联。
招募了40名痴呆症患者和40名对照者。对18份样本进行了使用16S rRNA测序的粪便微生物群分析。采用混合方法进行有力解读。
痴呆症患者的血清AG和AG/UAG比值升高。随着痴呆症患者中AG的增加，观察到物种丰富度显著下降。 以及 导致了β多样性的显著差异。 与简易精神状态检查表（MMSE）相关， 与蒙特利尔认知评估（MoCA）量表相关。
这项初步研究表明AG、肠道微生物群和认知评分之间存在复杂的相互作用。AG升高与痴呆症和肠道生态失调均相关，错综复杂地连接了肠-脑轴。循环AG及相关肠道微生物群可能是痴呆症的一种假定生物标志物。
