Wang Qin, Hao Wenwen, Guo Chao, Cao Hui, Wang Beiqi, Li Xingyang, Yu Ruilian, Xu Li, Li Jing
Wuxi Institute of Inspection, Testing and Certification, Wuxi, Jiangsu, China.
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
Appl Microbiol Biotechnol. 2025 Feb 10;109(1):40. doi: 10.1007/s00253-025-13412-6.
Human skin wounds primarily heal through reparative wound healing without pilosebaceous units or other appendages, rather than regenerative wound healing. Hair follicle (HF) regeneration is a significant challenge for skin wound healing. The effects and underlying mechanisms of Isaria cicadae Miquel rice fermentation extract (IMFRE) remain unclear, although it has anti-inflammatory, antioxidant, and reparative effects on oxidative damage in keratinocytes. We assessed the regenerative wound healing ability of IMFRE and its related molecular mechanisms through experimental validation and network pharmacology analysis. Our findings suggest that IMFRE could be an important potential solution for regenerative wound healing of skin hair follicle by utilizing the Hippo pathway regulatory mechanism. KEY POINTS: • IMFRE was found to significantly enhance the wound healing rate of mouse skin. • CK15 and CD34 were significantly increased by high-dose IMFRE intervention. • IMFRE could inhibit EGFR, GPCR, and Integrin expression.
人类皮肤伤口主要通过无毛囊皮脂腺单位或其他附属器的修复性伤口愈合来愈合，而非再生性伤口愈合。毛囊（HF）再生是皮肤伤口愈合面临的一项重大挑战。尽管蝉拟青霉大米发酵提取物（IMFRE）对角质形成细胞的氧化损伤具有抗炎、抗氧化和修复作用，但其作用效果及潜在机制仍不明确。我们通过实验验证和网络药理学分析评估了IMFRE的再生性伤口愈合能力及其相关分子机制。我们的研究结果表明，通过利用Hippo信号通路调控机制，IMFRE可能是皮肤毛囊再生性伤口愈合的一个重要潜在解决方案。要点：• 发现IMFRE可显著提高小鼠皮肤的伤口愈合率。• 高剂量IMFRE干预可使CK15和CD34显著增加。• IMFRE可抑制表皮生长因子受体（EGFR）、G蛋白偶联受体（GPCR）和整合素的表达。
