Major of Biomedical Engineering, Division of Smart Healthcare and New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea.
Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea; Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea.
Biomater Adv. 2022 Sep;140:213046. doi: 10.1016/j.bioadv.2022.213046. Epub 2022 Jul 25.
An extracellular matrix-mimicking, biodegradable tissue-engineered skin substitute with improved antibacterial, antibiofilm, and wound healing capabilities is essential in skin tissue regeneration applications. The purpose of this study was to develop a novel biodegradable composite nanofibrous poly(ε-caprolactone) (PCL)/decellularized extracellular matrix (dECM) scaffolds loaded with usnic acid (UA); (PEU), where UA is employed as an antibacterial agent as well as a wound-healing accelerator. The architecture and fiber structure of the scaffolds were examined using scanning electron microscopy, and the results revealed that the average diameters decreased as the dECM content increased. The chemical composition, changes in the crystalline structure, homogeneity, and thermal stability of the nanofiber scaffolds with different material compositions were determined using Fourier-transform infrared spectroscopy, X-ray diffraction, differential scanning calorimetry, and thermogravimetric analysis, respectively. The composite nanofibrous scaffolds exhibited strong antibacterial activity against various bacterial species, such as Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, and Cutibactrium acnes, and fungal pathogens (such as Candida albicans). Additionally, the composite nanofibrous scaffolds exhibited biofilm inhibition properties against Klebsiella pneumoniae and Pseudomonas aeruginosa. An evaluation of the appearance of in vivo full-thickness excisional wounds treated with the composite nanofiber scaffolds, as well as a histological analysis of the wounds 21 days after surgery, revealed that treatment with nanofibrous PEU scaffolds enhanced wound healing. This study reveals that the proposed composite nanofibrous PEU scaffold has substantial potential for treating infectious full-thickness wounds.
一种具有改善的抗菌、抗生物膜和伤口愈合能力的细胞外基质模拟、可生物降解的组织工程皮肤替代物对于皮肤组织再生应用至关重要。本研究旨在开发一种新型可生物降解的复合纳米纤维聚己内酯（PCL）/脱细胞细胞外基质（dECM）支架，负载有松萝酸（UA）；（PEU），其中 UA 用作抗菌剂和伤口愈合促进剂。使用扫描电子显微镜检查支架的结构和纤维结构，结果表明，随着 dECM 含量的增加，平均直径减小。使用傅里叶变换红外光谱、X 射线衍射、差示扫描量热法和热重分析分别确定了具有不同材料组成的纳米纤维支架的化学组成、晶体结构变化、均一性和热稳定性。复合纳米纤维支架对金黄色葡萄球菌、表皮葡萄球菌、变形链球菌和痤疮丙酸杆菌等各种细菌以及真菌病原体（如白色念珠菌）表现出强烈的抗菌活性。此外，复合纳米纤维支架对肺炎克雷伯菌和铜绿假单胞菌表现出抑制生物膜的特性。对用复合纳米纤维支架治疗的体内全厚切除伤口的外观进行评估，并对手术后 21 天的伤口进行组织学分析，结果表明，纳米纤维 PEU 支架治疗可促进伤口愈合。这项研究表明，所提出的复合纳米纤维 PEU 支架在治疗感染性全厚伤口方面具有很大的潜力。
