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一种用于植物基因组编辑的高效mRNA递送系统。

An efficient mRNA delivery system for genome editing in plants.

作者信息

Qiu Fengti, Xue Chenxiao, Liu Jinxing, Li Boshu, Gao Qiang, Liang Ronghong, Chen Kunling, Gao Caixia

机构信息

Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.

出版信息

Plant Biotechnol J. 2025 Apr;23(4):1348-1358. doi: 10.1111/pbi.14591. Epub 2025 Feb 10.

DOI:10.1111/pbi.14591
PMID:39928528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11933840/
Abstract

Transgene-free genome editing is important for crop improvement as it reduces unanticipated genomic changes. While mRNA delivery systems offer a powerful method for achieving transgene-free genome editing, they remain inefficient and challenging in plants. Here we describe an efficient mRNA delivery system for plants with substantially improved editing efficiency. By optimizing the 5' untranslated regions (5'UTRs) and poly(A) tails of in vitro-transcribed (IVT) mRNAs and coating the mRNA with protamine during particle bombardment, we have developed an optimized mRNA delivery system termed v2_TMV/DEN2. This system enhanced the efficiencies of knock-out, A-to-G and C-to-T base editing by an average 4.7-, 3.4- and 2.5-fold at various endogenous sites compared with plasmid-based transient delivery system via particle bombardment in rice suspension cells and wheat immature embryos 48 h post-transformation. Furthermore, we obtained edited plants with efficiencies of 5.0-180.8% and 26.1-26.2% using v2_TMV/DEN2 in rice and wheat, respectively, compared with 0.0-43.2% and 4.7-10.4% using plasmids. Our study provides a convenient and efficient mRNA delivery system for transgene-free genome editing in plants.

摘要

无转基因基因组编辑对于作物改良很重要，因为它能减少意外的基因组变化。虽然mRNA递送系统为实现无转基因基因组编辑提供了一种强大的方法，但它们在植物中仍然效率低下且具有挑战性。在此，我们描述了一种用于植物的高效mRNA递送系统，其编辑效率有了显著提高。通过优化体外转录（IVT）mRNA的5'非翻译区（5'UTR）和聚腺苷酸尾巴，并在粒子轰击过程中用鱼精蛋白包裹mRNA，我们开发了一种优化的mRNA递送系统，称为v2_TMV/DEN2。与在水稻悬浮细胞和小麦未成熟胚转化后48小时通过粒子轰击的基于质粒的瞬时递送系统相比，该系统在各种内源位点将敲除、A到G和C到T碱基编辑的效率平均提高了4.7倍、3.4倍和2.5倍。此外，使用v2_TMV/DEN2，我们在水稻和小麦中分别获得了效率为5.0 - 180.8%和26.1 - 26.2%的编辑植株，而使用质粒时的效率分别为0.0 - 43.2%和4.7 - 10.4%。我们的研究为植物无转基因基因组编辑提供了一种方便且高效的mRNA递送系统。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e26/11933840/e6ea5ca713ec/PBI-23-1348-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e26/11933840/95067a50a368/PBI-23-1348-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e26/11933840/7946ffb85141/PBI-23-1348-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e26/11933840/b47ba73e15fd/PBI-23-1348-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e26/11933840/e6ea5ca713ec/PBI-23-1348-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e26/11933840/95067a50a368/PBI-23-1348-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e26/11933840/7946ffb85141/PBI-23-1348-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e26/11933840/b47ba73e15fd/PBI-23-1348-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e26/11933840/e6ea5ca713ec/PBI-23-1348-g003.jpg

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3
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4
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Nat Rev Genet. 2024 Sep;25(9):603-622. doi: 10.1038/s41576-024-00720-2. Epub 2024 Apr 24.
5
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7
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8
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9
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10
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Nat Plants. 2022 May;8(5):535-548. doi: 10.1038/s41477-022-01152-8. Epub 2022 May 16.