State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100101, China.
Sci China Life Sci. 2022 Apr;65(4):731-738. doi: 10.1007/s11427-021-1949-9. Epub 2021 Aug 16.
Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1 (TaGRF4-TaGIF1) increased regeneration and genome editing frequency in wheat. When we introduced synonymous mutation in the miR396 target site of TaGRF4, the resulting complex (mTaGRF4-TaGIF1) performed better than original TaGRF4-TaGIF1. Use of mTaGRF4-TaGIF1 together with a cytosine base editor targeting TaALS resulted in 2-9-fold increases in regeneration and transgene-free genome editing in 11 elite common wheat cultivars. Therefore, mTaGRF4-TaGIF1 will undoubtedly be of great value in crop improvement and especially in commercial applications, since it greatly increased the range of cultivars available for transformation.
基因组编辑是一项前所未有的技术突破，但植物再生频率低和基因型依赖性阻碍了其在作物改良中的应用。在这里，我们发现瞬时表达生长调节剂 TaGRF4 和 TaGIF1 的复合物（TaGRF4-TaGIF1）可提高小麦的再生和基因组编辑频率。当我们在 TaGRF4 的 miR396 靶位点引入同义突变时，产生的复合物（mTaGRF4-TaGIF1）比原始 TaGRF4-TaGIF1 表现更好。使用 mTaGRF4-TaGIF1 与靶向 TaALS 的胞嘧啶碱基编辑器一起使用，可使 11 个优良普通小麦品种的再生和无转基因基因组编辑分别增加 2-9 倍。因此，mTaGRF4-TaGIF1 在作物改良中，特别是在商业应用中具有巨大的价值，因为它大大增加了可用于转化的品种范围。
