Wang Qi, Veley Kira M, Johnson Joshua M B, Sumner Josh, van Erven Gijs, Kabel Mirjam A, Dhungana Singha, Berry Jeffrey, Boyher Adam, Braun David M, Vermerris Wilfred, Bart Rebecca S
Donald Danforth Plant Science Center, St. Louis, MO 63132, U.S.A.
Laboratory of Food Chemistry, Wageningen University and Research, 6708 WG, Wageningen, The Netherlands.
Mol Plant Microbe Interact. 2025 May;38(3):400-410. doi: 10.1094/MPMI-05-24-0051-R. Epub 2025 Mar 13.
With an increasing demand for renewable fuels, bioenergy crops are being developed with high sugar content and altered cell walls to improve processing efficiency. These traits may have unintended consequences for plant disease resistance. pv. (), the causal agent of sorghum bacterial leaf streak, is a widespread bacterial pathogen. Here, we show that expresses several bacterial cell wall degrading enzymes (CWDEs) during sorghum infection, and these are required for full virulence. In tolerant sorghum, infection results in the induction of a key enzyme in monolignol biosynthesis, (), but this did not affect lignin content nor composition. Mutation of rendered the tolerant genotype susceptible. encodes caffeic acid -methyltransferase (COMT), an enzyme that generates sinapaldehyde as its major product. Growth inhibition of in the presence of sinapaldehyde was observed in vitro. We conclude that mutations that alter the components of the sorghum cell wall can reduce sorghum resistance to and that CWDEs contribute to bacterial virulence. Given the enhanced bioprocessing characteristics of sorghum, these results provide a cautionary tale for current and future efforts aimed at developing dedicated bioenergy crops. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.
随着对可再生燃料的需求不断增加，正在培育含糖量高且细胞壁经过改良的生物能源作物，以提高加工效率。这些特性可能会对植物抗病性产生意想不到的后果。高粱细菌性条斑病的病原菌（）是一种广泛存在的细菌病原体。在这里，我们表明在感染高粱期间表达了几种细菌细胞壁降解酶（CWDEs），而这些酶是完全致病力所必需的。在耐受性高粱中，感染会诱导单木质醇生物合成中的一种关键酶（），但这并不影响木质素含量和组成。的突变使耐受性基因型变得易感。编码咖啡酸 -甲基转移酶（COMT），一种以生成芥子醛为主要产物的酶。在体外观察到在芥子醛存在下的生长受到抑制。我们得出结论，改变高粱细胞壁成分的突变会降低高粱对的抗性，并且的CWDEs有助于细菌致病力。鉴于高粱增强的生物加工特性，这些结果为当前和未来致力于开发专用生物能源作物的努力提供了一个警示故事。[公式：见正文] 版权所有© 2025作者。这是一篇根据知识共享署名4.0国际许可协议分发的开放获取文章。
