Pasaribu Syarifah Aini, Basyuni Mohammad, Purba Edison, Hasanah Yaya
Unit Research Sungei Putih, Indonesian Rubber Research Institute, Galang, Deliserdang, North Sumatra, 20585, Indonesia.
Center of Excellence for Mangrove, Universitas Sumatera Utara, Medan, 20155, Indonesia.
F1000Res. 2023 Dec 4;12:106. doi: 10.12688/f1000research.129421.3. eCollection 2023.
Drought stress is one of the main causes of plant death. Strategies for plants survival are morphological adaptations, specific signaling pathways, and tolerance mechanisms. Rubber plantations have many uses, such as foreign exchange sources, job sources, forest revitalization, and a source of alternative wood for building materials and furniture. The rubber plant's response to drought stress is a complex biological process. A tolerant rubber clone in a dry area is the right approach. The present study aimed to identify drought tolerant traits in order to select or identify drought-tolerant clones at juvenile stage.
The first factor examined for this work was clones (IRR 425, IRR 428, IRR 429, IRR 434, IRR 440, RRIC 100, and BPM 24) and the second factor was water content (30%, 60%, and 90%). The study was arranged on a factorial randomized block design and repeated three times. Characteristics observed were total sugar (µM), proline (mg/L), chlorophyll a, b, total (µg/mL), hydrogen peroxidase (µmol/g), ascorbate peroxidase (unit/mg), superoxide dismutase (unit/mg), and peroxide dismutase (unit/mg).
The tolerance ability of the IRR 400 series rubber clones to drought stress was determined by observing the characteristics of sugar total and proline. The concentration of total sugar and proline were higher when the plant was treated with a lower water content. The selected clones tolerant to drought stress are RR 425 and IR 434 with high total sugar content and proline. Other characteristics, namely chlorophyll a, b, and total, as well as hydrogen peroxidase, ascorbate peroxidase, super oxide dismutase, peroxide dismutase, cannot be used as selection characteristics for this study.
This drought study of IRR 400 clones with varying water content percentages illustrated that the total sugar and proline characteristics could be used to distinguish tolerance levels from other observed characteristics.
干旱胁迫是植物死亡的主要原因之一。植物的生存策略包括形态适应、特定信号通路和耐受机制。橡胶种植园有多种用途，如外汇来源、就业来源、森林振兴以及建筑材料和家具的替代木材来源。橡胶树对干旱胁迫的反应是一个复杂的生物学过程。在干旱地区培育耐旱橡胶克隆品种是正确的方法。本研究旨在识别耐旱性状，以便在幼龄阶段选择或鉴定耐旱克隆品种。
本研究考察的第一个因素是克隆品种（IRR 425、IRR 428、IRR 429、IRR 434、IRR 440、RRIC 100和BPM 24），第二个因素是含水量（30%、60%和90%）。该研究采用析因随机区组设计，并重复三次。观察的特征包括总糖（微摩尔）、脯氨酸（毫克/升）、叶绿素a、b、总量（微克/毫升）、过氧化氢酶（微摩尔/克）、抗坏血酸过氧化物酶（单位/毫克）、超氧化物歧化酶（单位/毫克）和过氧化物歧化酶（单位/毫克）。
通过观察总糖和脯氨酸的特征来确定IRR 400系列橡胶克隆品种对干旱胁迫的耐受能力。当植物用较低含水量处理时，总糖和脯氨酸的浓度较高。所选的耐旱克隆品种是总糖含量和脯氨酸含量高的RR 425和IR 434。其他特征，即叶绿素a、b和总量，以及过氧化氢酶、抗坏血酸过氧化物酶、超氧化物歧化酶、过氧化物歧化酶，不能用作本研究的选择特征。
这项对不同含水量百分比的IRR 400克隆品种的干旱研究表明，总糖和脯氨酸特征可用于区分与其他观察到的特征不同的耐受水平。
