Bae Dahyun, Kim Minhee, Choi Jin-Sil
Department of Chemical and Biological Engineering, Hanbat National University Daejeon 34158 Korea
RSC Adv. 2025 Feb 10;15(6):4573-4580. doi: 10.1039/d5ra00047e. eCollection 2025 Feb 6.
Nanozymes have attracted attention owing to their distinct catalytic capabilities and potential applications, being advantageous compared to natural enzymes in terms of storage and cost efficiency. In this study, we investigated the enzymatic properties of iron oxide nanoclusters (IOCs) formed through the clustering of small nanoparticles. Our findings reveal that the enzymatic activity of IOCs is enhanced as their size increases. Additionally, we demonstrated that the size of the unit particles within IOCs is highly dependent on the nucleation environment, which is a crucial factor in determining the overall size of the IOCs. Importantly, the surface area of IOCs is more closely related to the size of the individual unit particles rather than the entire cluster. Smaller unit particle sizes within IOCs resulted in an increase in nanocluster size, thereby augmenting the specific surface area. The optimal IOC exhibited superior stability under various conditions and a broader range of reactivity compared to natural enzymes, making it a promising probe material for point-of-care tests across diverse environments. Furthermore, its effectiveness as a glucose detection probe was demonstrated, highlighting its potential for practical applications. The remarkable enzyme-like efficacy of IOCs not only enhances their utility in on-site detection technologies but also establishes them as a versatile detection probe.
纳米酶因其独特的催化能力和潜在应用而受到关注，在储存和成本效益方面比天然酶更具优势。在本研究中，我们研究了通过小纳米颗粒聚集形成的氧化铁纳米簇（IOCs）的酶学性质。我们的研究结果表明，IOCs的酶活性随着其尺寸的增加而增强。此外，我们证明了IOCs内单位颗粒的尺寸高度依赖于成核环境，这是决定IOCs整体尺寸的关键因素。重要的是，IOCs的表面积与单个单位颗粒的尺寸更密切相关，而不是与整个簇相关。IOCs内较小的单位颗粒尺寸导致纳米簇尺寸增加，从而增大了比表面积。与天然酶相比，最佳的IOC在各种条件下表现出优异的稳定性和更广泛的反应性，使其成为不同环境下即时检测的有前途的探针材料。此外，还证明了其作为葡萄糖检测探针的有效性，突出了其实际应用潜力。IOCs卓越的类酶功效不仅增强了它们在现场检测技术中的实用性，还确立了它们作为多功能检测探针的地位。
