Abdelfattah Yasmeen M, Mahmoud Amr M, Abdelaziz Noha I, El Mously Dina A
School of Pharmacy, Newgiza University, Km. 22 Cairo-Alex Road, P.O. Box 12577, Giza, Egypt.
School of Pharmacy, Newgiza University, Km. 22 Cairo-Alex Road, P.O. Box 12577, Giza, Egypt; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, El-Kasr-El Aini Street, Cairo, 11562, Egypt.
Anal Chim Acta. 2025 Jun 22;1356:343993. doi: 10.1016/j.aca.2025.343993. Epub 2025 Apr 4.
The applications of natural enzymes are vast, limited only by their protein nature. Therefore, the development of artificial enzyme mimetics, nanozymes, which are stable and have improved activity, has become indispensable for biomedical and diagnostic purposes. Nanozymes have developed into an emergent topic combining nanotechnology and biology due to their vast range of potential uses. In comparison to natural peroxidase, peroxidase-imitating nanozymes have distinct benefits in terms of high stability and low cost for applications in bioanalysis and environmental remediation. The use of metal-organic framework nanoparticles has exhibited enhanced catalytic and enzymatic performance.
In the current work, we present a strategy for synthesizing 2D Ni/Co MOF nanoparticles that have been anchored onto carboxymethyl cellulose (CMC). The resulting composite (Ni/Co-MOF@CMC) 2D nanosheets exhibit a high surface area and abundant catalytic sites, greatly amplifying their peroxidase-like catalytic performance. Additionally, these 2D bimetallic MOFs mimic the peroxidase activity, demonstrated by the distinctive yellow colour upon the oxidation of o-Phenylenediamine (OPD) by hydrogen peroxide. This newly synthesized 2D bimetallic MOF provides a straightforward, simple, selective, and sensitive colorimetric analysis technique for the determination of hydrogen peroxide and glucose. HO could be efficiently detected with a linear range of 10 μM-800 μM and a lower detection limit of 3.28 μM. With the potential to detect minute glucose concentrations as low as 200 μM within a linear range of 200 μM-600 μM.
This work demonstrates the significant novelty of applying an RGB colour sensor (TCS34725) for the quantitative measurement of HO and glucose which holds great potential as a point-of-care platform for diabetic patients. Consequently, our approach broadens the use of MOFs in biosensing and presents a viable substitute for affordable, and easily accessible diabetes monitoring. These 2D bimetallic MOFs are promising materials for glucose detection applications, expanding the utility of MOFs to include biosensor applications.
天然酶的应用广泛，但仅受其蛋白质性质的限制。因此，开发稳定且活性提高的人工酶模拟物——纳米酶，对于生物医学和诊断目的而言已变得不可或缺。由于其广泛的潜在用途，纳米酶已发展成为一个将纳米技术与生物学相结合的新兴课题。与天然过氧化物酶相比，模仿过氧化物酶的纳米酶在生物分析和环境修复应用中具有高稳定性和低成本的明显优势。金属有机框架纳米颗粒的使用已展现出增强的催化和酶促性能。
在当前工作中，我们提出了一种合成锚定在羧甲基纤维素（CMC）上的二维Ni/Co金属有机框架纳米颗粒的策略。所得复合材料（Ni/Co-MOF@CMC）二维纳米片具有高表面积和丰富的催化位点，极大地增强了它们的类过氧化物酶催化性能。此外，这些二维双金属金属有机框架模拟过氧化物酶活性，通过过氧化氢氧化邻苯二胺（OPD）时独特的黄色得以证明。这种新合成的二维双金属金属有机框架为过氧化氢和葡萄糖的测定提供了一种直接、简单、选择性和灵敏的比色分析技术。过氧化氢能够在10μM - 800μM的线性范围内被有效检测，检测下限为3.28μM。能够在200μM - 600μM的线性范围内检测低至200μM的微量葡萄糖浓度。
这项工作展示了应用RGB颜色传感器（TCS34725）对过氧化氢和葡萄糖进行定量测量的重大创新，作为糖尿病患者的即时检测平台具有巨大潜力。因此，我们的方法拓宽了金属有机框架在生物传感中的应用，并为经济实惠且易于获取的糖尿病监测提供了一种可行的替代方案。这些二维双金属金属有机框架是用于葡萄糖检测应用的有前途的材料，将金属有机框架的用途扩展到生物传感器应用领域。
