人呼吸道合胞病毒10种蛋白质的特性：一种新的与包膜相关的蛋白质的鉴定。

Characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein.

作者信息

Huang Y T, Collins P L, Wertz G W

出版信息

Virus Res. 1985 Mar;2(2):157-73. doi: 10.1016/0168-1702(85)90246-1.

Abstract

A total of 13 respiratory syncytial (RS) virus specific polypeptides were identified by pulse-chase metabolic labeling of infected HEp-2 cells. Ten of the 13 proteins were shown to be unique. They were the L, G, F (F1, F2), N, P, M, 24K, 14K, 11K and 9.5K proteins. These conclusions were based on peptide mapping and on previous work showing that each of 10 polypeptides are coded for by a unique mRNA. The seven largest proteins, L, G, F (F1, F2), N, P, M and 24K were identified clearly as virion structural proteins. The 24K protein was characterized by detergent and salt dissociation studies as an envelope-associated protein, bringing to four (G, F (F1, F2), M and 24K) the number of membrane associated proteins for RS virus. A fourth membrane-associated protein has not been described previously for any other paramyxovirus. Of the three smallest proteins, the 14K and 11K were characterized as non-structural proteins. The 9.5K protein was detected in low amounts in highly purified preparations of virions.

摘要

通过对感染的HEp-2细胞进行脉冲追踪代谢标记，共鉴定出13种呼吸道合胞（RS）病毒特异性多肽。13种蛋白质中有10种被证明是独特的。它们是L、G、F（F1、F2）、N、P、M、24K、14K、11K和9.5K蛋白。这些结论基于肽图谱分析以及先前的研究，该研究表明10种多肽中的每一种都由独特的mRNA编码。七种最大的蛋白质，即L、G、F（F1、F2）、N、P、M和24K，被明确鉴定为病毒粒子结构蛋白。通过去污剂和盐解离研究，24K蛋白被表征为一种包膜相关蛋白，使呼吸道合胞病毒的膜相关蛋白数量达到四种（G、F（F1、F2）、M和24K）。此前尚未在任何其他副粘病毒中描述过第四种膜相关蛋白。在三种最小的蛋白质中，14K和11K被表征为非结构蛋白。在高度纯化的病毒粒子制剂中，9.5K蛋白的含量较低。