呼吸道合胞病毒的多肽
Polypeptides of respiratory syncytial virus.
作者信息
Levine S
出版信息
J Virol. 1977 Jan;21(1):427-31. doi: 10.1128/JVI.21.1.427-431.1977.
Abstract
Radiolabeled respiratory syncytial virus was purified from medium that had been harvested from infected HeLa cell monolayers before it contained much cellular debris. After isopycnic centrifugation in linear gradients prepared with sucrose dissolved in Hanks balanced salt solution, almost all the infectivity and most of the radioactivity were recovered in a single band with density from 1.16 to 1.23 g/cm3 and a peak at 1.2 g/cm3. Analysis by polyacrylamide gel electrophoresis resolved the purified virus into seven polypeptides of approximate molecular weights 20,000 to 80,000, of which the two largest and the smallest proved to by glycoproteins.
摘要
放射性标记的呼吸道合胞病毒是从感染的HeLa细胞单层收获的培养基中纯化得到的，此时培养基中还没有太多细胞碎片。在用溶解于汉克斯平衡盐溶液中的蔗糖制备的线性梯度中进行等密度离心后，几乎所有的感染性和大部分放射性都在密度为1.16至1.23 g/cm3的单一条带中回收，峰值为1.2 g/cm3。通过聚丙烯酰胺凝胶电泳分析，纯化的病毒可分解为七种分子量约为20,000至80,000的多肽，其中最大的两种和最小的被证明是糖蛋白。
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