Kuroiwa T, Cahn R, Juhler M, Goping G, Campbell G, Klatzo I
Acta Neuropathol. 1985;66(1):3-11. doi: 10.1007/BF00698288.
The relationship between extravasation of proteins into extracellular spaces of brain parenchyma and the water content of such regions were evaluated in an experimental model. In this model, a temporary opening of the blood-brain barrier (BBB) to proteins was produced without significant injury to the cellular elements of brain tissue. Rabbits were subjected to bolus injection of their own blood under 360-400 mm Hg pressure via the internal carotid artery. The opening of the barrier and its duration were evaluated with Evans blue (EB), horseradish peroxidase (HRP), and sodium fluorescein (NaFl) tracers. The water content of brain tissue was assessed by specific gravity (SG) measurements in 1-mm-diameter tissue samples. Quantitative evaluation of protein penetration into brain tissue was carried out using 125I bovine serum albumin (BSA). The opening of the BBB to proteins persisted up to 9 h, whereas the barrier remained permeable to small molecular NaFl for 24 h. The SG measurements indicated in the areas of EB extravasation a progressive increment in water content up to 9 h, i.e., the duration of BBB opening to proteins. Following this, there was a progressive clearance of edema in spite of the BBB remaining open for NaFl for 24 h. Quantitative evaluations of 125I-BSA and SG in the same tissue samples, supported by statistical analysis, indicated approximately linear relationship analysis, indicated approximately linear relationship between albumin and water, implying a strong correlation between the development of vasogenic edema and extravasation of proteins into extracellular spaces.
在一个实验模型中评估了蛋白质外渗到脑实质细胞外间隙与这些区域含水量之间的关系。在该模型中，使血脑屏障（BBB）对蛋白质暂时开放，同时不对脑组织的细胞成分造成显著损伤。通过颈内动脉在360 - 400 mmHg压力下对兔子进行自体血液的大剂量注射。使用伊文思蓝（EB）、辣根过氧化物酶（HRP）和荧光素钠（NaFl）示踪剂评估屏障的开放情况及其持续时间。通过对直径1 mm的组织样本进行比重（SG）测量来评估脑组织的含水量。使用125I牛血清白蛋白（BSA）对蛋白质渗入脑组织进行定量评估。BBB对蛋白质的开放持续长达9小时，而屏障对小分子NaFl保持通透24小时。SG测量表明，在EB外渗区域，含水量在长达9小时内逐渐增加，即BBB对蛋白质开放的持续时间。在此之后，尽管BBB对NaFl保持开放24小时，但水肿逐渐消退。对同一组织样本中125I - BSA和SG的定量评估，经统计分析支持，表明白蛋白与水之间存在近似线性关系，这意味着血管源性水肿的发展与蛋白质外渗到细胞外间隙之间存在很强的相关性。
