Bodsch W, Hossmann K A
J Neurochem. 1983 Jul;41(1):239-43. doi: 10.1111/j.1471-4159.1983.tb11833.x.
The regional distribution of albumin in serum extravasations of cerebral edema was visualized on intact brain slices by autoradiography of 125I-labeled antibodies directed against albumin. Following autoradiographic imaging of edema protein spread, concentrations of total serum proteins were determined by radioimmunoassay in tissue micro samples taken from various regions of the brain. Peptide fragments of albumin--produced upon proteolytic breakdown of the native protein in vivo--were separated by affinity chromatography and HPLC. The combination of techniques for imaging, direct quantification, and analysis of molecular structure of serum proteins was provided to be valid in three different types of experimental cerebral edema in the rat: cortical cryogenic lesion, brain tumors, and stroke-prone spontaneous hypertension. The results indicate differences in the reactivity of edematous tissue with respect to proteolytic activity, depending on the susceptibility of serum proteins to in vivo fragmentation.
通过针对白蛋白的125I标记抗体的放射自显影术，在完整的脑切片上观察到脑水肿血清外渗中白蛋白的区域分布。在对水肿蛋白扩散进行放射自显影成像后，通过放射免疫测定法测定从大脑各个区域采集的组织微量样本中的总血清蛋白浓度。体内天然蛋白经蛋白水解产生的白蛋白肽片段通过亲和色谱法和高效液相色谱法分离。成像、直接定量和血清蛋白分子结构分析技术的组合被证明在大鼠的三种不同类型的实验性脑水肿中有效：皮质低温损伤、脑肿瘤和易中风的自发性高血压。结果表明，根据血清蛋白对体内片段化的敏感性，水肿组织在蛋白水解活性方面的反应性存在差异。
