Johnsen H, Ringvold A, Blika S
Acta Ophthalmol (Copenh). 1985 Feb;63(1):31-4. doi: 10.1111/j.1755-3768.1985.tb05211.x.
High-performance liquid chromatography on a Supelcosil LC-HN2 analytical column in weak anion exchange mode has allowed separation of ascorbic acid, in less than 5 min, from other interfering substances in serum and aqueous humour. UV monitoring at 254 nm enables ascorbic acid to be detected at 20 pmol level. A method for determination of ascorbic acid concentration in serum and aqueous humour is described, and values from 10 cataract patients are reported.
在弱阴离子交换模式下，使用Supelcosil LC-HN2分析柱进行高效液相色谱分析，能够在不到5分钟的时间内将抗坏血酸与血清和房水中的其他干扰物质分离。在254nm处进行紫外监测可在20皮摩尔水平检测到抗坏血酸。本文描述了一种测定血清和房水中抗坏血酸浓度的方法，并报告了10例白内障患者的检测值。
