Müller R M, Grundin T G, Roomans G M
Acta Physiol Scand. 1985 Apr;123(4):383-91. doi: 10.1111/j.1748-1716.1985.tb07604.x.
The effects of a single dose of reserpine or isoproterenol on the acinar cells of rat submandibular gland were investigated by electron microscopy and X-ray microanalysis. About 4 h after injection of reserpine, the cellular Ca concentration started to increase and at 24 h a level two to three times the control value was reached. The mucus content of the cells increased in parallel with the Ca concentrations. Isoproterenol stimulation depleted the acinar cells of their mucus. Resynthesis of mucus was visible at 8-12 h after injection. Changes in cellular Ca concentrations followed the mucus content of the cells. After 24 h both the mucus content and the Ca concentration were significantly increased over the control values. High-resolution X-ray microanalysis on thin cryosections showed that the calcium was accumulated in the mucus granules, both after reserpine and after isoproterenol administration. The newly synthesized mucus was also more calcium-rich than the original mucus. The effect of a single isoproterenol stimulation on elemental content of rat submandibular gland acinar cells was not significantly different from that of chronic isoproterenol treatment. Chronic treatment with reserpine resulted in a higher cellular Na/K ratio than a single dose.
通过电子显微镜和X射线微分析研究了单剂量利血平或异丙肾上腺素对大鼠颌下腺腺泡细胞的影响。注射利血平约4小时后，细胞内钙浓度开始升高，24小时时达到对照值的两到三倍。细胞内黏液含量与钙浓度平行增加。异丙肾上腺素刺激使腺泡细胞内的黏液减少。注射后8 - 12小时可见黏液重新合成。细胞内钙浓度的变化与细胞内黏液含量相关。24小时后，黏液含量和钙浓度均显著高于对照值。对薄冰冻切片进行的高分辨率X射线微分析表明，利血平和异丙肾上腺素给药后，钙均积聚在黏液颗粒中。新合成的黏液也比原来的黏液富含更多钙。单次异丙肾上腺素刺激对大鼠颌下腺腺泡细胞元素含量的影响与慢性异丙肾上腺素处理无显著差异。利血平慢性处理导致细胞内钠/钾比高于单剂量处理。
