Mohammed Ameen Sameera Sh, Algethami Faisal, Omer Khalid M
Department of Chemistry, College of Science, University of Zakho, 42002, Zakho, Kurdistan Region, Iraq.
Department of Chemistry, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), P.O. Box 90950, 11623, Riyadh, Saudi Arabia.
Mikrochim Acta. 2025 Feb 11;192(3):146. doi: 10.1007/s00604-025-07024-0.
Manganese nanoparticles (Mn NPs) were successfully synthesized using a cost-effective and eco-friendly biogenic approach, with pinus brutia pine needles. The produced Mn NPs were thoroughly characterized using common spectroscopic and microscopic techniques. The Mn NPs exhibited remarkable oxidase-like activity, effectively catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to its oxidized form (oxTMB) over a broad temperature range. This robust catalytic performance highlights their versatility and stability under varying thermal conditions, making them suitable for diverse applications. Nitrite forms orange-yellow color via diazotization of the blue oxTMB. An image processing algorithm analyzes the image changes induced by addition of nitrite. Under optimized conditions, the visual-based platform demonstrated a broad linear response for NO₂⁻ detection, spanning 0.0-58.0 uM and the detection limit was 0.11 μM. The visual method demonstrated exceptional sensitivity and recovery for nitrite detection in food samples. Combining simplicity, cost-effectiveness, and eco-friendliness, this instrument-free approach ensures accurate, portable, and point-of-care nitrite detection, ideal for real-world food testing applications.
采用具有成本效益且环保的生物合成方法，以土耳其松针为原料成功合成了锰纳米颗粒（Mn NPs）。使用常见的光谱和显微镜技术对所制备的Mn NPs进行了全面表征。Mn NPs表现出显著的类氧化酶活性，在较宽的温度范围内能有效地催化3,3',5,5'-四甲基联苯胺（TMB）氧化为其氧化形式（oxTMB）。这种强大的催化性能突出了它们在不同热条件下的多功能性和稳定性，使其适用于多种应用。亚硝酸盐通过蓝色oxTMB的重氮化反应形成橙黄色。一种图像处理算法分析了添加亚硝酸盐引起的图像变化。在优化条件下，基于视觉的平台对NO₂⁻检测表现出广泛的线性响应，范围为0.0 - 58.0 μM，检测限为0.11 μM。该视觉方法在食品样品中亚硝酸盐检测方面表现出卓越的灵敏度和回收率。结合简单性、成本效益和环境友好性，这种无需仪器的方法确保了准确、便携且即时的亚硝酸盐检测，非常适合实际食品检测应用。
