Long Nana, Qiu Min, Zuo Youwei, Deng Hongping
Center for Biodiversity Conservation and Utilization, School of Life Sciences, Southwest University, Beibei, Chongqing, People's Republic of China.
School of Laboratory Medicine, Chengdu Medical College, Chengdu, Sichuan, People's Republic of China.
Infect Drug Resist. 2025 Feb 7;18:731-744. doi: 10.2147/IDR.S491358. eCollection 2025.
The purpose of this study was to evaluate the antibacterial activity and mechanism of linalool against Methicillin-resistant (MRSA).
The determination of the antibacterial activity of linalool against clinically isolated MRSA strains was based on the minimum inhibitory concentration (MIC) and growth curve analysis. Finally, the inhibition mechanism of linalool was elucidated through metabolomic analysis and molecular docking.
Among the isolated strains, penicillin resistance was found to be the highest, while resistance to daptomycin/quinupristin-dalfopristin, linezolid, vancomycin, tetracycline, telithromycin, and levofloxacin was not observed. The MIC range of linalool was 211.24-1.65 μg/mL, with MIC and MIC values of 13.2 μg/mL and 105.62 μg/mL, respectively. Metabolomic analysis revealed that linalool interferes with various substance metabolisms and energy metabolism in MRSA, with the glutathione pathway potentially being a key pathway affected by linalool. Molecular docking revealed that linalool exhibited good binding potential to the target glutathione.
This study suggests that linalool could be developed as a drug or preservative to inhibit MRSA growth.
本研究旨在评估芳樟醇对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌活性及作用机制。
基于最低抑菌浓度（MIC）和生长曲线分析来测定芳樟醇对临床分离的MRSA菌株的抗菌活性。最后，通过代谢组学分析和分子对接阐明芳樟醇的抑制机制。
在分离出的菌株中，发现对青霉素的耐药性最高，而未观察到对达托霉素/奎奴普丁-达福普汀、利奈唑胺、万古霉素、四环素、泰利霉素和左氧氟沙星的耐药性。芳樟醇的MIC范围为211.24 - 1.65μg/mL，MIC₅₀和MIC₉₀值分别为13.2μg/mL和105.62μg/mL。代谢组学分析表明，芳樟醇干扰MRSA中的各种物质代谢和能量代谢，谷胱甘肽途径可能是受芳樟醇影响的关键途径。分子对接显示芳樟醇对靶标谷胱甘肽具有良好的结合潜力。
本研究表明芳樟醇可开发成为抑制MRSA生长的药物或防腐剂。
