School of Food Sciences & Engineering, Hainan University, 58 People Road, Haikou 570228, PR China.
School of Food Sciences & Engineering, Hainan University, 58 People Road, Haikou 570228, PR China.
Int J Food Microbiol. 2022 Oct 16;379:109846. doi: 10.1016/j.ijfoodmicro.2022.109846. Epub 2022 Jul 22.
Pseudomonas fragi is primarily responsible for the spoilage of various foods, especially meat. The aim of this study was to investigate the antibacterial mechanism of 3-carene against P. fragi. 3-Carene treatment decreased the phospholipid content and the fluidity of the cell membrane, induced reactive oxygen species (ROS) generation and affected respiratory chain dehydrogenase, oxoglutarate dehydrogenase and citrate synthase in P. fragi. Metabolomics and proteomics analyses further showed that in the presence of 3-carene, 519 proteins, 136 metabolites in positive ion mode and 100 metabolites in negative ion mode were differentially expressed. These proteins and metabolites were primarily involved in amino acid metabolism, fatty acid degradation, the tricarboxylic acid cycle (TCA cycle) and other processes. Consequently, the stimulation of 3-carene altered cell membrane properties, disturbed important amino acid and energy metabolism, and even caused oxidative stress. Additionally, the results of total viable counts and the total volatile base nitrogen indicated that 3-carene could significantly improve the preservation of refrigerated pork. This study suggested that 3-carene has promising potential to be developed as a food preservative.
弗氏柠檬酸杆菌主要负责各种食物，尤其是肉类的腐败。本研究旨在探讨 3-蒈烯对弗氏柠檬酸杆菌的抑菌机制。3-蒈烯处理降低了细胞膜的磷脂含量和流动性，诱导活性氧（ROS）的产生，并影响弗氏柠檬酸杆菌的呼吸链脱氢酶、氧化戊二酸脱氢酶和柠檬酸合酶。代谢组学和蛋白质组学分析进一步表明，在 3-蒈烯存在的情况下，有 519 种蛋白质、136 种正离子模式下的代谢物和 100 种负离子模式下的代谢物表达差异。这些蛋白质和代谢物主要参与氨基酸代谢、脂肪酸降解、三羧酸循环（TCA 循环）和其他过程。因此，3-蒈烯的刺激改变了细胞膜的性质，扰乱了重要的氨基酸和能量代谢，甚至导致氧化应激。此外，总活菌数和总挥发性碱基氮的结果表明，3-蒈烯可以显著改善冷藏猪肉的保存。本研究表明，3-蒈烯具有开发为食品防腐剂的巨大潜力。
