Gan Honghao, Chu Jianmin, Sun Jia, Wang Qian
Coastal Forestry Research Center, National Forestry and Grassland Administration, Beijing, 100091, China.
Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.
Plant Cell Rep. 2025 Feb 12;44(3):53. doi: 10.1007/s00299-025-03446-5.
High P increased the tolerance of R. pseudoacacia roots to salt stress. Salt is an important abiotic factor that restricts plant growth and development in soil. An appropriate concentration of P can increase plant tolerance to salt stress. We investigated the physiological and transcriptional regulatory effects of high P (HP) or low P (LP) on the response of R. pseudoacacia roots to salt stress. A pot experiment was carried out to grow R. pseudoacacia seedlings in vermiculite media supplemented with 0 mM, 150 mM or 300 mM NaCl under HP or LP conditions. The root dry weight and concentrations of free proline, P, ions, and phytohormones were measured, and the transcription of the genes was analyzed under NaCl stress under HP or LP conditions. The results revealed that R. pseudoacacia responds to NaCl stress by regulating the absorption and utilization of P and the levels of free proline, phytohormones and Na, K, Ca, and Mg as well as changing the expression levels of key genes. Compared with those under the LP condition, the roots of the R. pseudoacacia under the HP condition presented greater P concentrations, lower JA concentrations, and more stable K levels when subjected to NaCl stress, which increased their tolerance to NaCl stress. Moreover, genes involved in the cell wall, root growth, root architecture regulation, biomass accumulation, stress response, osmotic regulation and ion balance maintenance were upregulated under NaCl stress under HP conditions. In addition, NaCl stress impairs N metabolism under LP conditions. Our findings provide new insights into the response of woody plants to salt stress under different P conditions and contribute to the development of scientific afforestation in saline-alkali areas.
高磷提高了刺槐根系对盐胁迫的耐受性。盐是限制土壤中植物生长发育的重要非生物因素。适宜浓度的磷可以提高植物对盐胁迫的耐受性。我们研究了高磷（HP）或低磷（LP）对刺槐根系响应盐胁迫的生理和转录调控作用。进行了盆栽试验，使刺槐幼苗在添加0 mM、150 mM或300 mM NaCl的蛭石培养基中在高磷或低磷条件下生长。测定了根干重、游离脯氨酸、磷、离子和植物激素的浓度，并分析了高磷或低磷条件下NaCl胁迫下基因的转录情况。结果表明，刺槐通过调节磷的吸收和利用、游离脯氨酸、植物激素以及钠、钾、钙和镁的水平，并改变关键基因的表达水平来响应NaCl胁迫。与低磷条件下相比，高磷条件下的刺槐根系在受到NaCl胁迫时表现出更高的磷浓度、更低的茉莉酸浓度和更稳定的钾水平，这提高了它们对NaCl胁迫的耐受性。此外，在高磷条件下的NaCl胁迫下，参与细胞壁、根系生长、根系结构调控、生物量积累、胁迫响应、渗透调节和离子平衡维持的基因上调。此外，在低磷条件下，NaCl胁迫会损害氮代谢。我们的研究结果为不同磷条件下木本植物对盐胁迫的响应提供了新的见解，并有助于盐碱地科学造林的发展。
