Robertson N P, Cain G D
Anal Biochem. 1985 Apr;146(1):103-7. doi: 10.1016/0003-2697(85)90402-6.
The monosaccharide content of the ethanol-soluble moiety of crude polysaccharide fractions of the tapeworm Hymenolepis diminuta and the blood fluke Schistosoma mansoni was investigated by gas-liquid chromatography of alditol acetate derivatives. A prominent, unusual peak was found, and mass spectrometry suggested identification as an aminotetrose, a four-carbon aminosugar reported only once in the past 15 years [D. A. Cumming, C. G. Hellerquist, and O. Touster (1981) J. Biol. Chem. 256, 7723-7726]. The unknown molecule was found to be tris(hydroxymethyl)aminomethane (Tris), whose acetylated derivative has a mass spectrum identical to that of an aminotetrose. The source of the Tris was traced to buffers used in conjunction with polysaccharide isolation.
通过对糖醇乙酸酯衍生物进行气液色谱分析，研究了微小膜壳绦虫和曼氏血吸虫粗多糖级分的乙醇可溶部分中的单糖含量。发现了一个突出的异常峰，质谱分析表明其鉴定为氨基四糖，这是一种四碳氨基糖，在过去15年中仅报道过一次[D. A. 卡明、C. G. 赫勒奎斯特和O. 图斯特（1981年）《生物化学杂志》256, 7723 - 7726]。发现未知分子是三（羟甲基）氨基甲烷（Tris），其乙酰化衍生物的质谱与氨基四糖的质谱相同。Tris的来源追溯到与多糖分离一起使用的缓冲液。
