Robertson N P, Oaks J A, Cain G D
Mol Biochem Parasitol. 1984 Jan;10(1):99-109. doi: 10.1016/0166-6851(84)90022-7.
Polysaccharides and other complex carbohydrates were released by proteolysis of the chloroform-methanol insoluble residue of 10 day-old worms and eggs of Hymenolepis diminuta. Gas-liquid chromatographic analysis of alditol acetate derivatives of monosaccharides released from the polysaccharides by hydrolysis revealed that in the 10 day-old worm, glucose was the most abundant sugar, followed by galactose, glucosamine, galactosamine, fucose and possibly rhamnose. Mannose was least abundant and xylose was absent. In the egg, glucose and galactose were equally abundant, followed by the same sugars found in 10 day-old worms, and xylose was present. Uronic acid was detected in both fractions by specific chemical tests. None of the saccharide material from eggs and worms was susceptible to degradation by Streptomyces hyaluronidase, chondroitinase AC, and slightly susceptible to chondroitinase ABC, as shown by electrophoretic analysis on composite 2.2% acrylamide-agarose slab gels and 4.5/12.5% polyacrylamide gels before and after enzymatic treatment. One of the gel-separable bands, however, was degradable by both nitrous acid and Flavobacterium heparinase. Both bands from eggs were degradable by nitrous acid. These results suggest that eggs contain heparin and/or heparan sulfate and perhaps dermatan sulfate and that 10 day-old worms also have these polyglycans but possibly not chondroitin sulfate or hyaluronic acid.
通过对微小膜壳绦虫10日龄虫体和虫卵的氯仿 - 甲醇不溶性残渣进行蛋白水解，释放出多糖和其他复合碳水化合物。对多糖水解后释放的单糖的乙酰糖醇乙酸酯衍生物进行气 - 相色谱分析表明，在10日龄虫体中，葡萄糖是含量最丰富的糖，其次是半乳糖、葡萄糖胺、半乳糖胺、岩藻糖，可能还有鼠李糖。甘露糖含量最少，且不存在木糖。在虫卵中，葡萄糖和半乳糖含量相当，其次是在10日龄虫体中发现的相同糖类，并且存在木糖。通过特定化学测试在两个组分中均检测到糖醛酸。如在酶处理前后在2.2％丙烯酰胺 - 琼脂糖复合平板凝胶和4.5/12.5％聚丙烯酰胺凝胶上进行的电泳分析所示，来自虫卵和虫体的糖类物质均不易被透明质酸链霉菌、软骨素酶AC降解，对软骨素酶ABC略有敏感性。然而，其中一条可通过凝胶分离的条带可被亚硝酸和黄杆菌属肝素酶降解。来自虫卵的两条带均可被亚硝酸降解。这些结果表明，虫卵含有肝素和/或硫酸乙酰肝素，可能还含有硫酸皮肤素，并且10日龄虫体也含有这些聚糖，但可能不含硫酸软骨素或透明质酸。
