三（羟甲基）氨基甲烷的某些有机酸盐在孵育步骤中对血浆肾素活性的影响。

Effect of some organic acid salts of tris(hydroxymethyl)aminomethane in the incubation step on plasma renin activity.

作者信息

Frazer M G, Pearson D E

出版信息

Clin Chim Acta. 1980 Jul 17;105(1):117-22. doi: 10.1016/0009-8981(80)90099-6.

Abstract

The tris(hydroxymethyl)aminomethane salts of the organic acids acetylsalicylic, phenoxyacetic, salicylic, benzoic, acetic, and maleic were used to buffer the pH of plasma between 7.4 and 7.7 in a 1 h incubation step of the procedure for the determination of plasma renin activity. When compared to the plasma renin activity determined with tris(hydroxymethyl)aminomethane hydrochloride or with no buffer, it was found that all of the tris(hydroxymethyl)aminomethane salts of the organic acids studied produced a statistically significant increase in plasma renin activity except the salt of acetic acid when compared to tris(hydroxymethyl)aminomethane hydrochloride. In comparison to tris(hydroxymethyl)aminomethane hydrochloride and unbuffered plasma the organic acid salts and their mean percent difference in plasma renin activity are as follows: tris(hydroxymethyl)aminomethane phenoxyacetate (101%, 105%); tris(hydroxymethyl)aminomethane acetylsalicylate (79%, 115%); tris(hydroxymethyl)aminomethane benzoate (70%, 107%); tris(hydroxymethyl)aminomethane salicylate (64%, 70%); tris(hydroxymethyl)aminomethane maleate (32%, 60%); and tris(hydroxymethyl)aminomethane acetate (--1.6%, 43%).

摘要

在测定血浆肾素活性的过程中，使用有机酸乙酰水杨酸、苯氧乙酸、水杨酸、苯甲酸、乙酸和马来酸的三（羟甲基）氨基甲烷盐在1小时孵育步骤中将血浆pH缓冲在7.4至7.7之间。与用盐酸三（羟甲基）氨基甲烷或不使用缓冲剂测定的血浆肾素活性相比，发现除乙酸盐外，所研究的所有有机酸的三（羟甲基）氨基甲烷盐与盐酸三（羟甲基）氨基甲烷相比，均使血浆肾素活性有统计学意义的增加。与盐酸三（羟甲基）氨基甲烷和未缓冲的血浆相比，有机酸的盐及其在血浆肾素活性方面的平均百分比差异如下：三（羟甲基）氨基甲烷苯氧乙酸盐（101%，105%）；三（羟甲基）氨基甲烷乙酰水杨酸盐（79%，115%）；三（羟甲基）氨基甲烷苯甲酸盐（70%，107%）；三（羟甲基）氨基甲烷水杨酸盐（64%，70%）；三（羟甲基）氨基甲烷马来酸盐（32%，60%）；以及三（羟甲基）氨基甲烷乙酸盐（-1.6%，43%）。