Shevela Dmitriy, Schröder Wolfgang P, Messinger Johannes
Department of Chemistry, Umeå University, Umeå, Sweden.
Department of Chemistry - Ångström Laboratory, Uppsala University, Uppsala, Sweden.
Methods Mol Biol. 2018;1770:197-211. doi: 10.1007/978-1-4939-7786-4_11.
This chapter compares two different techniques for monitoring photosynthetic O production: the widespread Clark-type O electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell, and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.
广泛使用的克拉克型氧电极和更为精密的膜进样质谱（MIMS）技术。我们描述了如何用商用克拉克型电池制作一个简单的MIMS膜进样装置，并概述了这两种技术的优缺点，以指导研究人员决定使用哪种方法。文中给出了测量氧释放速率以及确定每个活性光系统II反应中心叶绿素分子数量的实验方案和示例。
