Liqi Zhu, Yuanyuan Liang, Linghan Yang, Jun Yin, Tao Wang, Quan Zhang
College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou, China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China.
Arch Microbiol. 2025 Feb 12;207(3):55. doi: 10.1007/s00203-025-04254-x.
The increasing attention given to the potential risk offered by enterohepatic Helicobacter species to the well-being of human beings and animals is of significant importance. Helicobacter mastomyrinus (H. mastomyrinus), a bacterium predominantly associated with rodents, has been implicated in liver and intestinal pathologies. Here, a strain of H. mastomyrinus, designated as Hm-17 (GenBank: CP145316.1), was isolated from asymptomatic C57BL/6 mice. Subsequently, an in-depth and comprehensive investigation was undertaken, which included genome sequencing analysis, micro-biochemical identification, evaluation of growth characteristic, cytotoxicity assessment, and testing of animal pathogenicity. The analysis of 16 S rRNA reveals a close phylogenetic relationship between H. mastomyrinus and H. canadensis. However, core-pan genome analysis and an evaluation of pathogenic factors indicates a more robust association between H. mastomyrinus and H. hepaticus. Cytotoxicity analysis revealed that Hm-17 exhibits robust cytolethal distending toxin (CDT) activity, inducing pronounced cellular swelling and death. Furthermore, Hm-17 infection in BALB/c mice results in rapid and characteristic focal necrotic hepatitis. Genome sequencing and pathogenicity analysis indicate that H. mastomyrinus isolates from asymptomatic mice possess significant pathogenic potential. These findings underscore the need for further investigation into the epidemiology and mechanisms of pathogenesis associated with this organism.
对肝肠螺杆菌属给人类和动物健康带来的潜在风险给予越来越多的关注具有重要意义。啮齿类动物螺杆菌（H. mastomyrinus），一种主要与啮齿动物相关的细菌，已被认为与肝脏和肠道病变有关。在此，从无症状的C57BL/6小鼠中分离出一株啮齿类动物螺杆菌，命名为Hm-17（GenBank：CP145316.1）。随后，进行了深入而全面的调查，包括基因组测序分析、微生物生化鉴定、生长特性评估、细胞毒性评估和动物致病性测试。16S rRNA分析显示啮齿类动物螺杆菌与加拿大螺杆菌之间存在密切的系统发育关系。然而，核心-泛基因组分析和致病因素评估表明，啮齿类动物螺杆菌与肝螺杆菌之间的关联更为紧密。细胞毒性分析表明，Hm-17表现出强大的细胞致死性扩张毒素（CDT）活性，诱导明显的细胞肿胀和死亡。此外，BALB/c小鼠感染Hm-17会导致快速且典型的局灶性坏死性肝炎。基因组测序和致病性分析表明，从无症状小鼠中分离出的啮齿类动物螺杆菌具有显著的致病潜力。这些发现强调了有必要进一步调查与这种生物体相关的流行病学和发病机制。
