Dos Santos Aniúsca Vieira, da Rocha Ana Carolina Monteiro, Dos Santos Giovana Tavares, Vieira Igor Araujo, de Oliveira Cáren Nunes, Basgalupp Suelen, Pedrotti Luana Giongo, Roglio Vinicius Serafini, de Brito Emerson Silveira, Dornelles Thayane Martins, Pereira Gerson Fernando Mendes, de Souza Flávia Moreno Alves, Wendland Eliana Marcia
Hospital Moinhos de Vento, PROADI - SUS, Porto Alegre, Brazil.
Graduate Program in Health Sciences, Federal University of Health Science of Porto Alegre, Porto Alegre, Brazil.
Sci Rep. 2025 Feb 12;15(1):5243. doi: 10.1038/s41598-025-88329-1.
Capillary blood collection presents advantages such as reduced invasiveness over venous serum for syphilis diagnosing. This study aimed to compare diagnostic accuracy between capillary and venous blood samples for syphilis diagnosis. Individuals aged ≥ 18 years were included in a cross-sectional study. Syphilis screening was done using Rapid tests (RT) followed by collection of serum capillary and venous samples for VDRL and TPHA test. Sensitivity, specificity, and Kappa coefficient were calculated. Of 191 participants, 115 RT + and 76 RT-. Diagnostic properties did not significantly differ between capillary and venous samples. Capillary VDRL showed 99% sensitivity and 100% specificity, mirroring TPHA results. Furthermore, there was significant agreement between sample types for both serological tests (p < 0.001). Capillary sampling offers comparable diagnostic accuracy to venous collection, regardless of serum quality. Capillary sampling holds promise, particularly in developing countries and large-scale testing initiatives.
与静脉采血检测梅毒血清相比，毛细血管采血具有侵入性较小等优点。本研究旨在比较毛细血管血样和静脉血样在梅毒诊断中的诊断准确性。年龄≥18岁的个体被纳入一项横断面研究。使用快速检测（RT）进行梅毒筛查，随后采集血清毛细血管血样和静脉血样进行性病研究实验室玻片试验（VDRL）和梅毒螺旋体血凝试验（TPHA）。计算敏感性、特异性和kappa系数。在191名参与者中，115名RT阳性，76名RT阴性。毛细血管血样和静脉血样之间的诊断特性没有显著差异。毛细血管VDRL显示出99%的敏感性和100%的特异性，与TPHA结果相符。此外，两种血清学检测的样本类型之间存在显著一致性（p<0.001）。无论血清质量如何，毛细血管采血与静脉采血具有相当的诊断准确性。毛细血管采血很有前景，特别是在发展中国家和大规模检测项目中。
