凋亡小泡的脂质代谢通过调节巨噬细胞功能加速皮肤伤口愈合。

Lipid metabolism of apoptotic vesicles accelerates cutaneous wound healing by modulating macrophage function.

作者信息

Qin Yuan, Chen Xin, Bao Lili, Ren Lili, Dou Geng, Lian Jianing, Xing Shujuan, Li Zihan, Ding Feng, Qin Wen, Liu Xulin, Zhu Bin, Liu Shiyu, Jin Zuolin, Yang Xiaoshan

机构信息

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Oral Biology & Clinic of Oral Rare Diseases and Genetic Disease, School of Stomatology, The Fourth Military Medical University, Xi' an, 710032, China.

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, 710032, China.

出版信息

J Nanobiotechnology. 2025 Feb 12;23(1):106. doi: 10.1186/s12951-025-03194-4.

DOI:10.1186/s12951-025-03194-4

PMID:39939963

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11823102/

Abstract

The application of apoptotic extracellular vesicles (ApoEVs) derived from stem cell in skin wound healing has garnered significant attention. In recent decades, scholars have shown that extracellular vesicles (EVs) established intercellular communication by carrying proteins or microRNAs, the role of lipids in EVs in wound healing has yet to be clarified. Here, we focus on the key role of group X secretory phospholipase A (sPLA-X) in lipid metabolism. Specifically, sPLA-X significantly increased the production of the anti-inflammatory lipid mediators, resolvin D5 (RvD5), by hydrolyzing phospholipids in ApoEVs. This change not only promoted the uptake of ApoEVs by macrophages, but also effectively inhibited the expression of tumor necrosis factor-alpha (TNF-α) in macrophages, promoting the healing of skin wounds. In summary, this study contributes to our understanding of the mechanisms by which ApoEVs support skin defect repair and offers a potential theoretical approach for using ApoEVs in skin wound treatment. With further research and optimization, it is expected that more efficient and secure ApoEVs-based treatment strategies will be developed, bringing new breakthroughs in clinical treatment of skin injuries and related diseases.

摘要

源自干细胞的凋亡细胞外囊泡（ApoEVs）在皮肤伤口愈合中的应用已引起广泛关注。近几十年来，学者们发现细胞外囊泡（EVs）通过携带蛋白质或微小RNA建立细胞间通讯，然而EVs中的脂质在伤口愈合中的作用尚未阐明。在此，我们聚焦于X组分泌型磷脂酶A（sPLA-X）在脂质代谢中的关键作用。具体而言，sPLA-X通过水解ApoEVs中的磷脂显著增加抗炎脂质介质解炎素D5（RvD5）的产生。这一变化不仅促进了巨噬细胞对ApoEVs的摄取，还有效抑制了巨噬细胞中肿瘤坏死因子-α（TNF-α）的表达，促进了皮肤伤口的愈合。总之，本研究有助于我们理解ApoEVs支持皮肤缺损修复的机制，并为在皮肤伤口治疗中使用ApoEVs提供了潜在的理论方法。随着进一步的研究和优化，有望开发出更高效、安全的基于ApoEVs的治疗策略，为皮肤损伤及相关疾病的临床治疗带来新的突破。