Department of Stomatology, People's Hospital of Longhua Shenzhen, Shenzhen, Guangdong, China.
State Key Laboratory of Oral Disease & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Oral Regenerative Medicine, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
J Nanobiotechnology. 2024 Sep 20;22(1):579. doi: 10.1186/s12951-024-02845-2.
The high incidence and mortality rates associated with acute and chronic wound infections impose a significant burden on global healthcare systems. In terms of the management of wound infection, the reconstruction and regeneration of skin appendages are essential for the recovery of mechanical strength and physiological function in the regenerated skin tissue. Novel therapeutic approaches are a requisite for enhancing the healing of infected wounds and promoting the regeneration of skin appendages. Herein, a novel antimicrobial microneedle patch has been fabricated for the transdermal controlled delivery of adipose tissue-derived apoptotic vesicles (ApoEVs-AT@MNP) for the treatment of infected wounds, which is expected to achieve high-quality scarless healing of the wound skin while inhibiting the bacteria in the infected wound. The microneedle patch (MNP) system possesses adequate mechanical strength to penetrate the skin, allowing the tips to remain inside tissue for continuous active release of biomolecules, and subsequently degrades safely within the host body. In vivo transplantation demonstrates that ApoEVs-AT@MNP not only inhibits bacterial proliferation in infected wounds but also significantly promotes effective and rapid scarless wound healing. Particularly noteworthy is the ability of ApoEVs-AT@MNP to promote the rapid formation of mature, evenly arranged hair follicles in infected wounds, observed as early as 8 days following implantation, which is essential for the restoration of skin function. This rapid development of skin appendages has not been reported this early in previous studies. Therefore, ApoEVs-AT@MNP has emerged as an excellent, painless, non-invasive, and highly promising treatment for infected wounds.
急性和慢性伤口感染的高发病率和死亡率给全球医疗保健系统带来了巨大负担。在伤口感染的管理方面，皮肤附属物的重建和再生对于恢复再生皮肤组织的机械强度和生理功能至关重要。新型治疗方法是增强感染性伤口愈合和促进皮肤附属物再生的必要条件。本文构建了一种新型抗菌微针贴片，用于经皮控释脂肪组织来源的凋亡小体（ApoEVs-AT@MNP），以治疗感染性伤口，有望实现伤口皮肤的高质量无疤痕愈合，同时抑制感染伤口中的细菌。微针贴片（MNP）系统具有足够的机械强度穿透皮肤，使针尖能够留在组织内持续主动释放生物分子，随后在宿主体内安全降解。体内移植表明，ApoEVs-AT@MNP 不仅抑制感染伤口中的细菌增殖，而且显著促进有效和快速的无疤痕伤口愈合。特别值得注意的是，ApoEVs-AT@MNP 能够促进感染伤口中成熟、均匀排列的毛囊的快速形成，在植入后 8 天即可观察到，这对于皮肤功能的恢复至关重要。以前的研究中尚未报道过这种皮肤附属物的快速发育。因此，ApoEVs-AT@MNP 已成为治疗感染性伤口的一种极好、无痛、非侵入性且极具前景的方法。
