Krauß Jakob, Georgieva Radostina, Karabaliev Miroslav, Hackmann Moritz, Rerkshanandana Pichayut, Chaiwaree Saranya, Kalus Ulrich, Pruß Axel, Xiong Yu, Bäumler Hans
Institute of Transfusion Medicine, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.
Department of Medical Physics, Biophysics & Radiology, Medical Faculty, Trakia University, 6000 Stara Zagora, Bulgaria.
Nanomaterials (Basel). 2025 Jan 24;15(3):187. doi: 10.3390/nano15030187.
The use of nanoparticles is becoming increasingly apparent in a growing number of medical fields. To exploit the full potential of these particles, it is essential to examine their behavior in the blood and their possible interactions with blood cells. Dendritic core multi-shell DendroSol™ nanoparticles (DS-NPs) are able to penetrate into viable layers of human skin, but nothing is known about their interaction with blood cells. In the present study, we analyze the effect of DS-NPs on red blood cells (RBCs) using confocal laser scanning microscopy (CLSM), flow cytometry, sedimentation rate analysis, spectrophotometry, and hemolysis assays. DS-NPs labeled with Nile red (NR) were added to RBC suspensions and their accumulation in the area of the RBC membranes was demonstrated by CLSM as well as by flow cytometry. In the presence of DS-NPs, the RBCs show an increased sedimentation rate, which also confirms the binding of the NPs to the cells. Interestingly, in the presence of DS-NPs, the RBCs are stabilized against hypotonic hemolysis as well as against the hemolytic action of Triton X-100. This proven anti-hemolytic effect could be utilized to enhance the circulation time of RBCs loaded with drugs for prolonged sustained release and drug delivery with enhanced bioavailability.
纳米颗粒在越来越多的医学领域中的应用正变得日益明显。为了充分发挥这些颗粒的潜力，研究它们在血液中的行为以及它们与血细胞可能的相互作用至关重要。树枝状核多壳层DendroSol™纳米颗粒（DS-NPs）能够穿透人皮肤的活性层，但关于它们与血细胞的相互作用却一无所知。在本研究中，我们使用共聚焦激光扫描显微镜（CLSM）、流式细胞术、沉降率分析、分光光度法和溶血试验来分析DS-NPs对红细胞（RBCs）的影响。将用尼罗红（NR）标记的DS-NPs添加到RBC悬浮液中，CLSM和流式细胞术均证实了它们在RBC膜区域的积累。在DS-NPs存在的情况下，RBC的沉降率增加，这也证实了纳米颗粒与细胞的结合。有趣的是，在DS-NPs存在的情况下，RBC对低渗溶血以及Triton X-100的溶血作用具有稳定性。这种已证实的抗溶血作用可用于延长载药RBC的循环时间，以实现延长的持续释放和提高生物利用度的药物递送。
